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  • Heliothis virescens (F)  (1)
  • Ultrastructure  (1)
  • 1
    Digitale Medien
    Digitale Medien
    GM1-gangliosidosis in a cross-bred dog confirmed by detection of GM1-ganglioside using electrospray ionisation-tandem mass spectrometry (2000)
    Springer
    Acta neuropathologica 100 (2000), S. 409-414 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words GM1-gangliosidosis ; Ultrastructure ; Electrospray ionisation tandem mass spectrometry ; Diagnosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The post-mortem diagnosis of lysosomal storage diseases can be confounded by the unavailability of suitable material. Here we report the diagnosis of GM1-gangliosidosis in a cross-bred dog, from which only formalin-fixed brain was available, by a combination of electron microscopy and the detection of elevated levels of GM1-ganglioside within the tissue using the novel technique of electrospray ionisation tandem mass spectrometry. Electron microscopic examination of ultrathin sections of resin-embedded tissue revealed cytoplasmic inclusions (membranous cytoplasmic and zebra bodies) in brain stem and cerebellar neurons that were characteristic of a gangliosidosis. Glycolipids were extracted from the fixed tissue and analysed by tandem mass spectrometry. Two major ions were detected, which corresponded to GM1 (d18:1–C18:0) and GM1 (d20:1–C18:0). Their identity was confirmed by comparison of their fragmentation patterns with those of authentic standards. The concentration of GM1 was approximately sixfold higher on a wet weight basis than in the brain of a normal control dog, confirming the diagnosis of GM1-gangliosidosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004010000187
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Effects of cuticular duvane diterpenes from green tobacco leaves on tobacco budworm (Lepidoptera: Noctuidae) oviposition (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 1349-1359 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Tobacco budworms ; Heliothis virescens (F) ; Lepidoptera ; Noctuidae ; tobacco ; Nicotiana tabacum L. ; diterpenes ; duvanes ; host plant resistance ; oviposition
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Five cuticular chemical components isolated from the green leaves of tobacco introductions (TIs) and a commercial tobacco cultivar were tested for their effects on tobacco budworm,Heliothis virescens (F), oviposition in cage bioassays, and field experiments. These chemicals were sprayed onto budworm-resistant TI 1112 tobacco which produces low levels of most cuticular components. Individual duvane diterpenes (α- and β-4,8,13-duvatrien-1-ols and α- and β-4,8,13-duvatriene-1,3-diols) increased tobacco budworm egg laying on sprayed TI 1112 plants.cis-Abienol, docosanol, and docosanyl myristate were inactive.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01012354
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