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Digitale Medien

Feasibility of simultaneous fluorescence immunophenotyping and fluorescence in situ hybridization study for the detection of estrogen receptor expression and deletions of the estrogen receptor gene in breast carcinoma cell lines (2000)

Zhang, Y. ; Siebert, R. ; Matthiesen, P. ; [weitere]

Springer

Virchows Archiv 436 (2000), S. 271-275

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ISSN: 1432-2307

Schlagwort(e): Key words Immunophenotyping ; FISH ; Breast carcinoma ; Deletions ; ESR gene

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract For the first time, combined immunophenotyping and fluorescence in situ hybridization (FISH) technique according to the ”fluorescence immunophenotyping and interphase cytogenetics as a tool for investigation of neoplasms” (FICTION) technique have been successfully applied in solid tumors. Thus, we were able to visualize the antigen expression of cells with chromosomal deletions of a tumor suppressor region directly. In six breast carcinoma cell lines, we investigated the correlation between estrogen receptor (ER) expression status and deletions of the estrogen receptor gene (ESR). To screen for deletions of the ESR gene, dual-color FISH was performed with a YAC (yeast artificial chromosome) probe containing the ESR gene and, as internal control, with a centromeric probe of chromosome 6. Deletions of the ESR gene were detected in four of six cell lines. For direct comparison of ER expression with the copy number of the ESR gene at the single cell level, immunophenotyping with mouse anti-human ER antibody was combined with FISH with the YAC probe containing the ESR gene according to the FICTION technique. There was no correlation between lack of or reduced ER expression and deletions of the ESR gene. One cell line with deletions of the ESR gene did express ER on the protein level, while another cell line without a deletion did not. Cells with deletions of the ESR gene were either ER expression positive or negative. The staining intensity of ER expression was not associated with the copy number of the ESR gene. Thus, this FICTION study unequivocally shows that deletions of the ESR gene are not the major cause of absent or reduced ER expression in breast carcinoma cell lines.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004280050040

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Digitale Medien

Über Reduktone (1937)

Micheel, F. ; Bode, G. ; Siebert, R.

Weinheim : Wiley-Blackwell

Berichte der deutschen chemischen Gesellschaft 70 (1937), S. 1862-1866

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ISSN: 0365-9631

Schlagwort(e): Chemistry ; Inorganic Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Zusätzliches Material: 1 Ill.