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Digitale Medien

Theory and practice of isoelectric focusing of interacting systems (1998)

Cann, John R.

Weinheim : Wiley-Blackwell

Electrophoresis 19 (1998), S. 1577-1585

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Details

ISSN: 0173-0835

Schlagwort(e): Isoelectric focusing ; Interacting systems ; Theory and practice ; Computer simulations ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: The theory of mass transport coupled to reversible protein interactions forms the basis for computer simulation of the isoelectric focusing behavior of several model systems. These include pH-dependent conformational transition, carrier ampholyte-induced interactions and protein-ligand interactions. The computational results compare favorably with experimental observations. In addition, a method is formulated for an isoelectric focusing procedure which enables determination of intrinsic ligand-binding constants for statistical binding of a charged ligand, binding to heterogeneous sites, and cooperative binding.

Zusätzliches Material: 11 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150191010

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