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  • 1
    Digitale Medien
    Digitale Medien
    Comparison of two juvenile hormone radioimmunoassays (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 23 (1993), S. 147-152 
    Details
    ISSN: 0739-4462
    Schlagwort(e): honey bee ; Apis mellifera ; juvenile hormone ; radioimmunoassay ; hemolymph ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Juvenile hormone from the hemolymph of adult worker honey bees of known age and behavioral status was extracted and analyzed by two different radioimmunoassays in two independent laboratoies. The assays are different in hapten attachment, radiolabeled tracer, and the method by which bound and unbound hormone are separated. Despite these differences in the methods, hormone determinations were in excellent agreement at lower levels (0-50 ng/ml) but diverged as the hormone concentrations increased (〉 50 ng/ml). The relative changes are in good agreement, with a correlation coefficient of 0.97. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/arch.940230306
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Juvenile hormone regulation of hemolymph juvenile hormone binding protein in the black strain of the tobacco hornworm, Manduca sexta (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 30 (1995), S. 165-176 
    Details
    ISSN: 0739-4462
    Schlagwort(e): Lepidoptera ; radioimmunoassay ; enzyme immunoassay ; equilibrium dialysis ; monoclonal antibody ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Numerous studies have demonstrated regulation of specific lepidopteran proteins by pharmacological doses of insect juvenile hormone (JH). In this study, topical application of a 1 pg dose of JH I to fourth stadium larvae of the black (bl) mutant strain of the tobacco hornworm, Manduca sexta, induced a 50% increase in the titer of hemolymph juvenile hormone binding protein (hJHBP). Radioimmunoassay confirmed that JH titers were lower in bl larvae than in wild-type larvae at the time of JH treatment. Enzyme immunoassay analysis of hJHBP titers demonstrated that regulation by JH I was dose-dependent at doses up to 10 pg and that the response was saturated above 100 pg. Western blotting and equilibrium dialysis confirmed these results and demonstrated that hJHBP from bl larvae had the same molecular mass and displayed the same affinity for JH I as hJHBP isolated from wild-type larvae. Time course studies showed that regulation was complex: 1 2 h after JH I treatment, hJHBP titers were twofold lower in treated than in control bl larvae, while 44 h after treatment they were twofold higher. JH I regulation of hJHBP titers in bl larvae was independent of changes in total hemolymph protein. © 1995 Wiley-Liss, Inc.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/arch.940300207
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Recent advances in radioimmunoassay technology for the juvenile hormones (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 30 (1995), S. 295-306 
    Details
    ISSN: 0739-4462
    Schlagwort(e): radioimmunoassay ; JH I ; JH II ; JH III ; hemolymph ; insect hormone ; Manducasexta sexta ; Hyalophora cecropia ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Recent refinements in juvenile hormone radioimmunoassay technology now make this method significantly more sensitive and easier to use. Rabbit poly-clonal antisera against (10R) JH III and racemic JH II have been developed to determine hemolymph hormone titers in the low picogram range. The antisera display minimal cross-reactivity with JH metabolites, JH analogs, and hemolymph lipids. One antiserum recognizes racemic JH I, II, and (10R) III almost equivalently, exhibiting 50% displacement between 100 and 130 pg per tube. Another antiserum is JH II-specific and exhibits 50% displacement at 35 pg per tube. Assay sensitivity has been enhanced by using (10R,11S) [methyl-3H]-JH II of very high specific activity (〉 80 Ci/mmol) generated with Hyalophora cecropia accessory gland S-adenosylmethionine transferase and S-[methyl-3H]-adenosyl-L-methionine. Preparation of biological samples has been simplified with overall recoveries of JH from hemolymph ranging between 60 and 75%. © 1995 Wiley-Liss, Inc.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/arch.940300215
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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