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  • 1
    Digitale Medien
    Digitale Medien
    Induction of chromosome aberrations by ^9^0Sr β-particles in cultured human lymphocytes
    Amsterdam : Elsevier
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 163 (1986), S. 277-283 
    Details
    ISSN: 0027-5107
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0027-5107(86)90026-6
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  • 2
    Digitale Medien
    Digitale Medien
    Molecular bases for cystic fibrosis in the Sardinian population (1990)
    Springer
    Human genetics 〈Berlin〉 85 (1990), S. 415-415 
    Details
    ISSN: 1432-1203
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Cystic fibrosis (CF) is a relatively uncommon genetic disorder in the Sardinian population. In this study, we have defined the frequency of the most common CF mutation (ΔF508) and carried out a genotypephenotype correlation analysis in a group of 21 patients with CF and of Sardinian descent. We detected the ΔF508 mutation in 24 (57%) out of the 42 CF chromosome investigated. This mutation was found in the homozygous state in 9 patients and in the heterozygous state in 6 patients. The remaining 6 patients had other mutations. The ΔF508 mutation was associated only with the KM19/XV2c 2 1 haplotype. Genotype-phenotype correlation analysis did not give clear-cut results, probably because of the small number of patients investigated. However, out of the four patients with meconium ileus, three were homozygous and one was heterozygous for the ΔF508 mutation, confirming that the presence of ΔF508 or other severe mutations in the homozygous state is the prerequisite for the development of meconium ileus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02428287
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  • 3
    Digitale Medien
    Digitale Medien
    Production of interspecific somatic hybrid plants in the genus Medicago through protoplast fusion (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 792-797 
    Details
    ISSN: 1432-2242
    Schlagwort(e): M. sativa ; M. coerulea ; Protoplast electrofusion ; Somatic hybrids ; Morphological analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Symmetric somatic hybrid plants have been produced by electrofusion of leaf protoplasts of Medicago sativa and callus protoplasts of Medicago coerulea. The selection of hybrid individuals has been performed at the cellular level by recording the positions of single heterocaryons immobilized in a semisolid culture medium. The hybrid nature of the heterokaryons was assessed in fluorescent light on the basis of their color. Hybrid minicalli were picked up manually and grown first on propagating, and then on regenerating, media. Six putative hybrid calli were selected and two of them regenerated several plants. The hybrid nature of the regenerants was confirmed by cytological and isozyme analysis. Among the several morphological traits taken into account for the characterization of somatic hybrid plants, some were intermediate, some lower, and some higher, with respect to the parents. The somatic hybrid plants were fertile and set seed. The production of somatic hybrid plants in the genus Medicago is discussed in relation to the regenerating capability of parental protoplasts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00227386
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  • 4
    Digitale Medien
    Digitale Medien
    Plant regeneration from callus and protoplasts in Medicago polymorpha (1993)
    Springer
    Plant cell, tissue and organ culture 35 (1993), S. 49-57 
    Details
    ISSN: 1573-5044
    Schlagwort(e): callus induction ; M. polymorpha ; protoplast isolation ; plant regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Seventeen ecotypes of the wild species Medicago polymorpha adapted to a Sardinian (Italy) environment have been evaluated for their response to tissue culture. The accession Samughero-Albi was the more respondent for callus induction and, together with Usassai, showed the highest regeneration capacity on media containing 1 mg l-1 2iP and 0.1 mg l-1 IAA. The morphogenetic response was also affected by the explant source. The hypocotyl-derived-calli were the best regenerating tissues. Regenerated plantlets were difficult to root and it was possible to obtain plants with a well developed root system only after 5–7 weeks of culture on media containing 2iP and IAA both at 0.2 mg l-1. Mesophyll cells were the best protoplast yielding source but only those isolated from roots were able to divide and to regenerate plants. Results are discussed in relation to the genotype specificity for the morphogenetic response and the feasibility of using M. polymorpha in the somatic hybridization with M. sativa.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00043939
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