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Digitale Medien

Phleomycin resistance as a dominant selectable marker in CHO cells (1988)

Mulsant, Philippe ; Gatignol, Anne ; Dalens, Maurice ; [weitere]

Springer

Somatic cell and molecular genetics 14 (1988), S. 243-252

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ISSN: 1572-9931

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract The Tn5 and the Streptoalloteichus hindustanus (Sh) ble genes conferring resistance to bleomycin-phleomycin antibiotics have been cloned into a mammalian vector under the RSV-LTR promoter. The resulting plasmids, pUT506 and pUT507 respectively, were used to transfect CHO cells by either the calcium phosphate or the recently described polybrene-DMSO method. Phleomycin- or bleomycin-resistant clones arose with a higher frequency after transfection with pUT507, and pUT507 transfectants were more resistant to both antibiotics than pUT506 transfectants. Phleomycin resistance in pUT507 transfectants was stable and associated with integration of plasmid sequences in genomic DNA. The Sh ble gene, which confers a dominant phleomycin-resistance phenotype, should provide a useful transferable selectable marker in CHO cells as well as in other animal cell lines.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01534585

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Digitale Medien

Effects of phleomycin-induced DNA damage on the fission yeast Schizosaccharomyces pombe cell cycle (1995)

Belenguer, Pascale ; Oustrin, Marie-Louise ; Tiraby, Gérard ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Yeast 11 (1995), S. 225-231

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ISSN: 0749-503X

Schlagwort(e): fission yeast ; cell cycle ; phleomycin ; DNA damage ; Life and Medical Sciences ; Genetics

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: The effect of phleomycin, a bleomycin-like antibiotic, has been investigated in the fission yeast, Schizosaccharomyces pombe. We report that in response to phleomycin-induced DNA damage, growth was inhibited and S. pombe cells arrested in the G2-phase of the cell cycle. DNA repair mutants rad9 and rad17 did not arrest and were hypersensitive to phleomycin. Cell cycle mutants that entered mitosis without monitoring the completion of DNA replication also displayed an increased sensitivity to this DNA-damaging agent. Thus, phleomycin could be used as a tool in the fission yeast S. pombe model system for the study of DNA damage and cell cycle checkpoints, or as a new selective agent.

Zusätzliches Material: 4 Ill.