ISSN:
1432-1424
Schlagwort(e):
Key words: Taste — Chloride conductance — Lingual epithelium — Niflumic acid — Flufenamic acid — DIDS
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract. We used Ussing chamber measurements and whole-cell recordings to characterize a chloride conductance in rat lingual epithelium. Niflumic acid (NFA) and flufenamic acid (FFA), nonsteroidal anti-inflammatory aromatic compounds known to inhibit Cl− conductances in other tissues, reduced transepithelial short-circuit current (I sc ) in the intact dorsal anterior rat tongue epithelium when added from the serosal side, and reduced whole-cell currents in rat fungiform taste cells. In both Ussing chamber and patch-clamp experiments, the effect of NFA was mimicked by replacement of bath Cl− with methanesulfonate or gluconate. In low Cl− bath solution, the effect of NFA on whole-cell current was reduced. Replacement of bath Ca2+ with Ba2+ reduced the whole-cell Cl− current. We conclude that a Ca2+-activated Cl− conductance is likely present in the basolateral membrane of the rat lingual epithelium, and is present in the taste receptor cells from fungiform papillae. Further experiments will be required to identify the role of this conductance in taste transduction.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s002329900396
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