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  • 11
    Electronic Resource
    Electronic Resource
    Heat shock induces HIV-1 replication in chronically infected promyelocyte cell line OM10.1 (1996)
    Springer
    Archives of virology 141 (1996), S. 439-447 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A long period of clinical latency before development of symptoms is characteristic of human immunodeficiency virus type 1 (HIV-1) infection. OM10.1, a promyelocyte cell line latently infected with HIV-1, has been developed as a model for studying the mechanism of viral latency and the activation of virus expression. We found that this latently infected cell line with heat shock at 42°C for 2 h resulted in a high level of HIV-1 production without addition of any cytokines. The mechanism of activation was analyzed by using anti-TNF-α antibody and various inhibitors. Although the TNF-α level in culture supernatants was below the sensitivity of an ELISA assay system, addition of anti-TNF-α antibody in culture medium could partially suppress the heat shock induced HIV-1 production. Staurosporine (PKC inhibitor), pentoxifylline (NF-ϰB inhibitor), and Ro5-3335 (HIV-1 Tat inhibitor) also inhibited significantly the heat shock induced virus activation. In particular, staurosporine achieved approximately 90% inhibition of the HIV-1 antigen expression in heat shock-treated OM10.1 at a non-toxic concentration. Although the mechanism of HIV-1 activation with heat shock has not been fully elucidated yet, it is presumed PKC plays an important role in HIV-1 activation. Thus, the present observations will provide a further insight into the pathogenesis of HIV-1 infections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01718308
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    Paper (German National Licenses)
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  • 12
    Electronic Resource
    Electronic Resource
    Production method of no-carrier-added186Re (1996)
    Springer
    Journal of radioanalytical and nuclear chemistry 205 (1996), S. 85-92 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract No-Carrier-Added186Re was produced using the186W(p,n)186Re nuclear reaction with 13.6 MeV protons on thick targets of 99.79% isotopically enriched186WO3. The theoretical excitation functions for producing186Re, and possible radionuclidic impurities of182Re,183Re, and184Re were calculated using the ALICE code. Cross-sections of the186W(p,n)186Re reaction were measured up to 20 MeV using the stacked target method with thin foils of natural composition tungsten metal. The experimental and theoretical excitation functions were in good agreement. Targetry used at the TIARA cyclotron, and a radiochemical separation scheme for186Re are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02040553
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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