ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A precise analysis for the identification of the yeast genusSchizosaccharomyces by immunoenzymatic methods is presented. By use of the murine monoclonal antibody JHF13–17, two proteins were recognized in cell extracts ofS. pombe, S. malidevorans, andS. japonicus in Western blot analyses and were termed SSP-A and SSP-B (Schizosaccharomyces-specific proteins). The migration pattern of the proteins was identical in 18 strains of these species that were tested. An analogous protein doublet was also visualized in cell extracts of ten strains ofS. octosporus, and, owing to the migration pattern, these proteins were termed SSP-B and SSP-C. A polyclonal antiserum raised against the SSP-A and SSP-B fromS. pombe cross-reacted with SSP-A and SSP-B fromS. malidevorans andS. japonicus and SSP-B and SSP-C fromS. octosporus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02092151
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