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  • 11
    Digitale Medien
    Digitale Medien
    The alpha subunit of the mitochondrial ATP synthetase is mitochondrially made in the unicellular heterotrophPrototheca zopfiii (1985)
    Springer
    Current genetics 10 (1985), S. 125-131 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Prototheca zopfii ; Algal mitochondria ; Mitochondrial ATPase ; Mitochondrial genes ; Mitochondrial protein synthesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Prototheca zopfii is a unicellular, colorless alga which grows heterotrophically on several different carbon sources.P. zopfii cells were grown, labeled with [35S] sulfate in the presence of cycloheximide, and the mitochondrial fraction isolated and analyzed by gel electrophoresis and autoradiography. Cycloheximide resistant translation products included a heavily labeled protein with an apparent molecular weight of 60,000. This labeled protein co-fractionated with mitochondria when the mitochondria were purified on Percoll gradients. When F1-ATPase was isolated from the mitochondria, an active ATPase preparation was obtained which, when analyzed by gel electrophoresis, was found to contain 2 prominent proteins in the 55 kd to 60 kd region of the gel. Autoradiography of this electrophoretically-resolved ATPase preparation revealed that the larger of the two, bands, but not the smaller, was heavily labeled with [35S]. Extraction of ATP synthetase (F1 -F0) from mitochondria with the detergent octylglucoside, yielded a preparation containing, as major components, the same two 55 kd to 60 kd proteins seen in the F1-ATPase preparation. Limited proteolysis of the larger protein, isolated from mitochondria that had been labeled in the presence of cycloheximide, was carried out usingStaphylococcus aureus V8 protease or papain. The pattern of peptide fragments observed by Coomassie Blue staining was essentially identical to that observed by autoradiography. This shows that the labeled protein corresponds to the alpha subunit of the ATPase. Thus the alpha subunit of the mitochondrial ATP synthetase ofP. zopfii is synthesized in the mitochondria.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00636477
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  • 12
    Digitale Medien
    Digitale Medien
    Velocity structure and properties of the lunar crust (1972)
    Springer
    Earth, moon and planets 4 (1972), S. 490-504 
    Details
    ISSN: 1573-0794
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie , Physik
    Notizen: Abstract Lunar seismic data from three Apollo seismometers are interpreted to determine the structure of the Moon's interior to a depth of about 100 km. The travel times and amplitudes ofP arrivals from Saturn IV B and LM impacts are interpreted in terms of a compressional velocity profile. The most outstanding feature of the model is that, in the Fra Mauro region of Oceanus Procellarum, the Moon has a 65 km thick layered crust. Other features of the model are: (i) rapid increase of velocity near the surface due to pressure effects on dry rocks, (ii) a discontinuity at a depth of about 25 km, (iii) near constant velocity (6.8 km/s) between 25 and 65 km deep, (iv) a major discontinuity at 65 km marking the base of the lunar crust, and (v) very high velocity (about 9 km/s) in the lunar mantle below the crust. Velocities in the upper layer of the crust match those of lunar basalts while those in the lower layer fall in the range of terrestrial gabbroic and anorthositic rocks.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00562013
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  • 13
    Digitale Medien
    Digitale Medien
    Zytogenetik und molekulare Untersuchungen sichern die histopathologischen Diagnosen von chronischen myelopro-liferativen Erkrankungen ab (1995)
    Springer
    Der Pathologe 16 (1995), S. 41-45 
    Details
    ISSN: 1432-1963
    Schlagwort(e): Schlüsselwörter Chronische myeloproliferative Erkrankungen ; Philadelphia-Translokation ; Zytogenetik ; Molekulargenetik ; Fluoreszenz-in-situ-Hybridisierung ; Histopathologie ; Key words Chronic myeloproliferative disorders ; Philadelphia-translocation ; Cytogenetics ; Molecular genetics ; Fluorescence in situ hybridization ; Histopathology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Summary The histopathological classification of chronic myeloproliferative disorders can be supported by applying cytogenetics and molecular genetics to the analysis of bone marrow or blood cells, as demonstrated in 253 cases evaluated. The Philadelphia translocation (9;22) is the most important genetic parameter, being specific for chronic myeloid leukemia. Conventional methods for the detection of the t(9;22) are karyotyping and Southern blot analysis of the bcr gene. The newly established technique of fluorescence in situ hybridization (FISH) allows visualization of bcr-abl fusion even in non dividing cells. Molecular cytogenetics for t(9;22) yield results that are rapid and reliable as well as easily quantifiable.
    Notizen: Zusammenfassung Zytogenetische und molekulargenetische Untersuchungen von Knochenmark- oder Blutzellen sind für die histopathologische Klassifikation der chronischen myeloproliferativen Erkrankungen hilfreich, was durch die simultane Auswertung von 253 Fällen gezeigt wird. Insbesondere die Analyse der Philadelphia-Translokation (9;22) ist dabei für die Bestätigung oder den Ausschluß einer chronischen myeloischen Leukämie wichtig. Für den Nachweis der t(9;22) stehen die konventionelle Karyotypisierung mit Bestimmung des Philadelphia-Chromosoms und das Southernblotverfahren zur Analyse einer Umlagerung des bcr-Gens zur Verfügung. Durch die neuere Methode der Fluoreszenz-in-situ-Hybridisierung (FISH) kann auch eine bcr-abl-Fusion an Interphasekernen dargestellt werden. Diese molekulare Zytogenetik ist ein rasches und zuverlässiges Verfahren zum Nachweis der Philadelphia-Translokation, das zudem leicht quantifizierbare Ergebnisse liefert.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002920050074
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  • 14
    Digitale Medien
    Digitale Medien
    Identification and characterization of mitochondrial translation products in various yeasts and inPrototheca zopfii (1985)
    Springer
    Current genetics 9 (1985), S. 661-670 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Mitochondrial protein synthesis ; Yeast mitochondria ; Cytochrome oxidase ; Prothotheca zopfii
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Mitochondrial genomes of different eucaryotes are not all alike. We have examined mitochondrial translation products in a number of yeasts (Candida krusei, Hansenula saturnus, Rhodotorula glutinis, Rhodotorula rubra, Torulopsis glabrata andSaccharomyces cerevisiae) and in Prototheca zopfii, a colorless alga, in order to determine whether certain proteins are invariably synthesized within mitochondria, how different these proteins are, and what additional proteins, if any, might be synthesized by diverse mitochondria. Using a variety of techniques and criteria, including immunological analysis and peptide mapping, we show that all the yeasts studied, and probablyP. zopfii as well, make versions of the 3 large subunits of cytochrome c oxidase. Not all of these oxidase subunits are equally closely related to their counterparts inS. cerevisiae, however. Mitochondria of some of the yeasts studied do not make, or make only small amounts of, a counterpart to Varl, a major mitochondrially made protein inS. cerevisiae. Mitochondria ofP. zopfii possibly do not make an apocytochrome b.T. glabrata, H. saturnus and the two Rhodotorula species each make one or more proteins whose relationship, if any, to mitochondrial translation products ofS. cerevisiae is not apparent. These results provide new information about mitochondrial diversity. Whereas mitochondria of all the organisms that we have studied devote the major part of their synthetic effort to making the three large subunits of cytochrome c oxidase, and probably make certain other proteins in common, they do not all synthesize a completely identical set of proteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00449819
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  • 15
    Digitale Medien
    Digitale Medien
    Selection for acid tolerance inRhizobium meliloti (1988)
    Springer
    Plant and soil 105 (1988), S. 179-188 
    Details
    ISSN: 1573-5036
    Schlagwort(e): acid tolerance ; Rhizobium meliloti ; saprophytic competence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract In the selection of acid-tolerantRhizobium meliloti, procedures for the collection and isolation of rhizobia, and the assessment of acid tolerance, have not been critically evaluated. Such procedures form the basis of this study. Root nodules were collected fromMedicago spp. found growing on acid soil in Sardinia. Their encumbent bacteria were isolated directly onto media adjusted over a range of pH values, and then assessed for acid tolerance in both the laboratory and field. Strains ofRhizobium meliloti isolated onto low pH media were, in general, more acid-tolerant than sister isolates from high pH media, when tested in both the laboratory and field. Dilution (10 or 100 fold) of the inocula used in the laboratory assessment did not greatly influence the rating derived, although there was some effect of bacterial colony type on growth rating. The link between polysaccharide production and acid tolerance was not strong. There was a poor correlation between the growth ratings derived from the laboratory screening and acid tolerance as expressed in the field.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02376781
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  • 16
    Digitale Medien
    Digitale Medien
    The use of MES buffer in early nodulation studies with annual Medicago species (1991)
    Springer
    Plant and soil 131 (1991), S. 199-206 
    Details
    ISSN: 1573-5036
    Schlagwort(e): annual Medicago ; nodulation ; buffer ; nutrient solution ; pH stability ; 2-(N-morpholino)ethanesulfonic acid (MES)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The usefulness of 2-(N-morpholino)ethanesulfonic acid (MES) to stabilize the pH of solutions in the range pH 5.5 to 7.0 during early growth and nodulation of annual Medicago (medic) species was investigated. In the first experiment, effects of MES concentrations (0, 1, 2, 5, 10 mM) on growth and nodulation of Medicago polymorpha L. and the stability of solution pH were investigated. In the second experiment we assessed the effect of MES on the growth and nodulation of three medic species (M. truncatula Gaernt., M. polymorpha and M. murex Willd.) at a range of starting pH levels (5.5, 6.0, 6.5 and 7.0) with different concentrations of MES buffer (0, 0.5, 10 mM). In a third experiment, the effects of the concentration of extra cations and the species (K-ion or Na-ion), used as hydroxide to bring solutions containing different MES concentrations to target pH were investigated. MES had no effect on growth at MES concentrations as high as 10 mM but nodule numbers were increased by concentrations of MES of 2 mM or greater. The presence of extra K-ion or Na-ion in solutions had no effect on plant growth or nodulation. With low MES concentrations (0.5 mM), solution pH stability was improved by daily titration and the use of a high nitrate to ammonium ratio (9 : 1) in solution. This combination of strategies maintained pH within a narrow range without effects on annual medic growth or nodulation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00009449
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