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  • 11
    Electronic Resource
    Electronic Resource
    Chromatographic resolution of enantiomers using albumin as complexing agent in the mobile phase
    Amsterdam : Elsevier
    Journal of Pharmaceutical and Biomedical Analysis 4 (1986), S. 221-235 
    Details
    ISSN: 0731-7085
    Keywords: Resolution of enantiomers ; albumin as complexing agent ; carboxylic acids ; indirect UV detection. ; reversed-phase liquid chromatography
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0731-7085(86)80044-9
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  • 12
    Electronic Resource
    Electronic Resource
    The metabolism of 3'-azido-2',3'-dideoxyguanosine in CEM cells
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 166 (1990), S. 273-279 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(90)91941-K
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  • 13
    Electronic Resource
    Electronic Resource
    Nasal air sampling used for the assessment of occupational allergen exposure and the efficacy of respiratory protection (2002)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 32 (2002), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Occupational exposure to rodent allergens may cause laboratory animal allergy. Personal exposure to occupational allergens is measured by collecting airborne dust on filters using person-carried pumps. This technique cannot be used to evaluate personal protective respiratory equipment. Recently developed intranasal air samplers collect inhaled particles by impaction on adhesive strips within the samplers.Objective The aims were to compare rodent aeroallergen exposure assessment using nasal air samplers with personal air sampling, and to evaluate the efficacy of using respiratory protection during rodent work using nasal air samplers.Methods Aeroallergen exposure was assessed during rodent work using both nasal air samplers and personal air samplers. The efficacy of respiratory protection (P2 facemasks and fresh-air helmets) was studied in subject pairs working side by side, one person with protection, the other without. Right nostril samples were laminated with protein-binding membrane and immunostained for rat urinary allergen-containing particles. Left nostril samples and air samples were eluted in buffer and analysed in amplified ELISAs for rat (RUA) and mouse (MUA) urinary allergen content (detection limit 10 pg/mL). Allergen collection efficacy of the nasal air samplers was tested at high and low exposure levels and at different flow rates using static sampling.Results P2 facemasks decreased the amount of inhaled allergen by about 90%, and very little allergen was inhaled using fresh-air helmets. Allergen levels in air and nasal samples correlated well (rs was about 0.8 for both RUA and MUA). The number of RUA-positive particles and nasal allergen levels measured in ELISA also correlated significantly (rs = 0.8). Collection efficacy of the nasal air sampler was better during high exposure (cleaning cages, median 73% of allergenic particles collected), than during low exposure (undisturbed room, 49% of particles).Conclusion Nasal air sampling is a relevant and sensitive complement to personal air sampling and enables evaluation of personal respiratory protection equipment. Use of P2 facemasks and fresh-air helmets may substantially reduce occupational exposure to inhaled allergens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2002.01545.x
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  • 14
    Electronic Resource
    Electronic Resource
    Comparison of four allergen-sampling methods in conventional and allergy prevention classrooms (2002)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 32 (2002), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Several allergen-sampling methods are used to assess level of personal or indirect exposure to cat in homes, schools and other public buildings and working environments.Objective To compare four different allergen-sampling methods (dust collectors, Petri dishes, person-carried pumps and intranasal samplers) by simultaneous sampling in classrooms and to compare the cat allergen levels between conventional classrooms and allergy prevention classrooms. Another aim was to relate the results to self-reported frequency of allergy and asthma symptoms among the children, to their perception of the school environment.Methods Among all compulsory schools (n = 257) in the Stockholm suburban area, 35 classrooms (five with implemented allergy prevention measures, seven with additional cleaning and 23 with normal cleaning routines) were chosen for allergen-sampling. Dust collectors (two models), Petri dishes, person-carried pumps and intranasal samplers were used simultaneously. All children (n = 829) received a self-administered questionnaire which included questions about home and school environment, allergic disease, asthma symptoms and pet contact.Results The correlation between sampling methods was generally poor. Furthermore, there was no significant difference in allergen levels between allergy prevention and allergen avoidance classes compared to conventional classes. Median levels were generally, but not significantly, lower in classes with few cat owners, compared to classes with many cat owners. Children in allergy prevention classes were more satisfied with the indoor air quality and cleaning than children attending classes with fewer or no allergy prevention measures (P 〈 0.0001). Nine per cent of all children reported allergic symptoms while at school.Conclusion The lack of correlation between sampling methods used simultaneously demonstrates the difficulty in assessing allergen levels in schools and similar environments. The implemented intervention measures (allergy prevention/allergen avoidance) did not influence cat allergen levels at school.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2002.01553.x
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  • 15
    Electronic Resource
    Electronic Resource
    Lack of correlation between NADPH-oxidase priming and elevated alkaline phosphatase activity in cord blood neutrophils (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Pediatric allergy and immunology 6 (1995), S. 0 
    Details
    ISSN: 1399-3038
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Human neutrophils were isolated from cord blood drawn after Caesarean section deliveries without labour and from peripheral blood from healthy adults. The alkaline phosphatase (ALP) activity in the cell populations was compared with the release of respiratory burst products after activation with the chemoattractant n-formyl-methionyl-leucyl-phenylalanine (fMLP). In contrast to cord blood neutrophils obtained from babies after normal vaginal deliveries, the Caesarean section neutrophils were not metabolically primed. However, like the neutrophils from vaginally delivered babies, the Caesarean section neutrophils showed an increased ALP activity compared to adult control neutrophils. These findings indicate that priming of cord blood neutrophils appears to be induced by labour and can be dissociated from increased ALP activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3038.1995.tb00276.x
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  • 16
    Electronic Resource
    Electronic Resource
    Low-threshold operation of hemispherical microcavity single-quantum-well lasers at 4 K (1993)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 62 (1993), S. 443-445 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We demonstrate low-threshold lasing at 4 K in optically pumped hemispherical In0.2Ga0.8As single-quantum-well microcavities. The incident threshold pump power density is 11 kW/cm2 corresponding to an absorbed power density of about 320 W/cm2, and the measured spontaneous emission factor β is about 0.01.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.108928
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  • 17
    Electronic Resource
    Electronic Resource
    Phagocyte Activation by Trp–Lys–Tyr–Met–Val–Met, Acting through FPRL1/LXA4R, is not Affected by Lipoxin A4 (2002)
    Oxford, UK : Blackwell Science Ltd
    Scandinavian journal of immunology 56 (2002), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Lipoxin A4 (LXA4) has been shown to bind to the leucocyte formyl peptide receptor (FPR) homologue, FPRL1, without triggering the biological activities induced by other FPRL1 agonists. We investigated the direct effect of LXA4 as well as the effect on agonist-induced biological responses using transfected HL-60 cells expressing FPR, FPRL1 or FPRL2. LXA4 neither induced an intracellular rise in calcium in these transfectants nor affected the response induced by the peptide Trp–Lys–Tyr–Met–Val–Met (WKYMVM), an agonist that activates cells through FPRL1 and -2. Both agonists induced Erk-2 activation; however, the eicosanoid-induced activity was independent of FPRL1 and FPRL2. Moreover, LXA4 was unable to trigger neutrophil upregulation of complement receptor 3 and respiratory burst, and it had no effect on the responses induced by triggering with WKYMVM. We conclude that LXA4 is unable to affect the WKYMVM-induced signalling through FPRL1 and suggest that it acts through a receptor different from FPRL1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.2002.01149.x
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  • 18
    Electronic Resource
    Electronic Resource
    Phagocytosis following Translocation of the Neutrophil b-Cytochrome from the Specific Granule to the Plasma Membrane is Associated with an Increased Leakage of Reactive Oxygen Species (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 36 (1992), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effect of neutrophil b-cytochrome translocation on the respiratory burst activation generated during phagocytosis of yeast particles was investigated. Secretion of neutrophil specific granules was induced by the calcium ionophore ionomycin prior to phagocytosis. The secretory process is associated with a translocation from the specific granules to the plasma membrane of the respiratory burst b-cytochrome. Respiratory burst activity was measured as release of hydrogen peroxide in the absence of azide (extracellular leakage) and in the presence of azide (total produclion). The subcellular localization of the b-cytochrome was found to affect the extracellular release of hydrogen peroxide in that a plasma membrane localization was associated with a significantly increased release during phagocytosis. It should be pointed out, however, that most of the hydrogen peroxide, both in control and in ionomycin-treated cells, is produced intracellularly, probably in the phagosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1992.tb03151.x
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  • 19
    Electronic Resource
    Electronic Resource
    Certain Human Gpl20-HIV Antibodies React with Anti-CD4 Antibodies (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 27 (1988), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: HIV+ human sera contain antibodies against most HIV proteins, including the envelope glycoprotein Gpl20. Some of these antibodies may have an epitope that sterically resembles the CD4 region to which the Gp120 molecule binds. Amongst 58 HIV+ sera tested, we found three with the capacity to block the binding of anti-CD4 monoclonal antibodies to CD4+ cells. The serum with the highest blocking capacity was selected for further analysis. The inhibitor was shown to be an antibody that binds to the Gp120 molecule as well us to the anti-CD4 monoclonal T4.2. These CD4-mimicking antibodies were shown not to interfere with CD4-dependent reactions in vitro. Virus neutralizing experiments in vitro could not show any neutralizing effect with these antibodies alone. The HIV+ individual providing this antibody is still healthy, although HIV since 1983.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1988.tb02328.x
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  • 20
    Electronic Resource
    Electronic Resource
    A Prospective Study of Immunoglobulin Changes in Relation to Human Immunodeficiency Virus Infection in a Cohort of Homosexual Men (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 29 (1989), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Changes in IgG levels and patterns in association with human immunodeficiency virus, (HIV) infection were characterized in a prospective investigation by studying serum electrophoresis and antibodies to HIV at least once a year in 144 homo/bisexual (HS) men during a 4-year period. Initially, 25 men (17.4%) were HIV-positive, and this increased to 44 at the end of the study period. HIV-negative men had IgG levels of 11.2 ± 2.2 g/l. In the 19 HIV seroconverters the mean IgG level rose with time according to the equation IgG = 0.054×+ 12.56 (x= months after the last HIV-negative test). Oligoclonal IgG bands were found in three of the 19 seroconverters (16%) prior to conversion and in 2/16 (13%), 5/13 (38%), 7/11 (64%), and 3/8 (38%) 1,2, 3, and 4 years after conversion, respectively. Of the 25 initially HIV-positive men, five have developed acquired immunodeficiency syndrome (AIDS) during the 4.5 year follow-up. At the first investigation these five men had higher mean IgG levels (16.0 ± 2.7 g/l) than the 20 men who have remained healthy (14.6 ± 2.8 g/l), and all live had oligoclonal bands as compared to 9/20 of those who did not develop AIDS. Oligoclonal IgG bands reflect the IgG increase and seem to indciate long-standing HIV infection and a poor prognosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1989.tb01162.x
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