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  • 11
    Electronic Resource
    Electronic Resource
    Lack of association of the insulin gene region with Type 1 (insulin-dependent) diabetes mellitus in Japanese subjects (1994)
    Springer
    Diabetologia 37 (1994), S. 210-213 
    Details
    ISSN: 1432-0428
    Keywords: Key words Insulin gene, polymorphism, Type 1 (insulin-dependent) diabetes mellitus, Japanese, susceptibility.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Although the insulin gene region is implicated in susceptibility to Type 1 (insulin-dependent) diabetes mellitus in Caucasians, significance of this region to Type 1 diabetes in Japanese remains unclear because the class 1 alleles (shorter insertion) of the variable number of tandem repeat in the 5′ region of the insulin gene are predominant in both diabetic and non-diabetic subjects. The 5′ insulin gene polymorphism was analysed in 75 Japanese patients and 69 control subjects with a precise method using PvuII and a polymorphism specific probe, which enabled us to divide class 1 alleles into four subclasses. Allelic frequencies were not significantly different between Type 1 diabetic patients and control subjects. The polymorphism in the 3′ untranslated region of the insulin gene (1127/ PstI) was also analysed and found to be tightly linked to the 5′ insulin gene polymorphism, and thus was not associated with diabetes. Interaction between HLA-DR and the insulin gene region, which was reported in the French study, was not observed in Japanese. These results suggest that the insulin gene region is not a valuable genetic risk factor for Type 1 diabetes in Japanese. [Diabetologia (1994) 37: 210–213]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050095
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  • 12
    Electronic Resource
    Electronic Resource
    An immunohistochemical study of cytokeratin and glial fibrillary acidic protein in chroid plexus papilloma (1988)
    Springer
    Acta neuropathologica 75 (1988), S. 317-320 
    Details
    ISSN: 1432-0533
    Keywords: Cytokeratin ; Glial fibrillary acidic protein ; Choroid plexus ; Choroid plexus papilloma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cellular localization of cytokeratin and glial fibrillary acidic protein (GFAP) was examined in two normal choroid plexuses and five choroid plexus papillomas by the peroxidase-antiperoxidase (PAP) method and double immunofluorescence (IFL) microscopy. Cytokeratin was observed in the majority of epithelial cells in all samples of normal and neoplastic choroid plexuses. On the other hand, GFAP was observed in some of the constituent epithelial cells in two cases of papilloma. Most of these GFAP-positive papilloma cells were simultaneously positive for cytokeratin, as could be seen by the PAP stainings of serial sections and by the double IFL stainings of the same sections. From these findings, it was suggested that normal and neoplastic choroid plexus epithel cells usually express cytokeratin and that some of the neoplastic cells can simultaneously express boty cytokeratin and GFAP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690541
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  • 13
    Electronic Resource
    Electronic Resource
    An immunohistochemical analysis of T cells in primary B cell malignant lymphoma of the brain (1989)
    Springer
    Acta neuropathologica 79 (1989), S. 27-29 
    Details
    ISSN: 1432-0533
    Keywords: Malignant lymphoma ; Non-Hodgkin lymphoma ; Brain tumor ; Tumor-infiltrating lymphocyte ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An immunohistochemical study was performed on small lymphoid cells present in frozen tissue sections of seven cases of primary B cell malignant lymphomas of the brain by using monoclonal antibodies to T cell (Leu-1, OKT-11, Leu-3a, and Leu-2a) and B cell (BA-1 and Leu-12) surface markers. In all the seven cases, positive reaction for Leu-1 and OKT-11 was seen in the majority of the small lymphoid cells which were dispersed among the lymphoma cells or clustered around blood vessles. The large neoplastic cells were unstained by these antibodies. Staining for T cell subsets with antibodies to Leu-3a and Leu-2a showed heterogeneous staining in each case. The ratio of Leu-3a+ to Leu-2a+ cells was less than one in six cases, demonstrating a suppressor/cytotoxic phenotype predominance. Most of these small lymphoid cells were negatively stained by antibodies to BA-1 and Leu-12. From these findings, it was shown that the small lymphoid cells observed in primary B cell lymphomas of the brain were of T cell lineage, distinct from the neoplastic cells, and probably reactive in nature. The implications of these findings are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00308953
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  • 14
    Electronic Resource
    Electronic Resource
    Primary malignant lymphoma of the brain: An immunohistochemical study of eight cases using a panel of monoclonal and heterologous antibodies (1986)
    Springer
    Acta neuropathologica 71 (1986), S. 190-196 
    Details
    ISSN: 1432-0533
    Keywords: Malignant lymphoma ; Brain tumor ; Non-Hodgkin lymphoma ; Burkitt's lymphoma ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Frozen sections of eight primary malignant lymphomas of the brain were examined by the immunohistochemical methods using a panel of monoclonal and heterologous antibodies to B lymphocyte (immunoglobulins, BA-1, Leu-12 and HLA-DR), T lymphocyte (OKT-11 and Leu-1) and monocyte (OKM-1) surface markers. Paraffin sections were also used in the examination of cytoplasmic immunoglobulins. Surface and/or cytoplasmic immunoglobulins (Ig) were observed in seven cases,four of which were shown to be distinctly monoclonal and the other three less so. The remaining 1 case showed no distinct staining for Ig. BA-1, Leu-12 and HLA-DR stainings were positive in four, four and five cases, respectively. The marker phenotypes of (BA-1, Leu-12, HLA-DR) were shown to be (+,+,+) in one lymphoma, (+,-,-) in three, (-,+,+,)in three, and (-,-,+) in one. Thus, it was demonstrated that the present lymphoma cases showed a marked immunological heterogeneity, and it was shown that all of them including the Ig-negative case revealed one or more of these three additional B cell markers, indicating B cell lineage of these cases. Examination of T cell and monocyte markers revealed positive staining in normal or reactive lymphoid cells distributed around blood vessels or sporadically in tumor tissues, but not in lymphoma cells. Epstein-Barr virus (EBV)-associated nuclear antigen was not demonstrated in the seven cases examined, making it unlikely that these lymphomas were related with EBV infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688039
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  • 15
    Electronic Resource
    Electronic Resource
    An immunohistochemical study on “neoplastic angioendotheliosis”: demonstration of B lymphocyte markers in the neoplastic cells (1988)
    Springer
    Acta neuropathologica 75 (1988), S. 313-316 
    Details
    ISSN: 1432-0533
    Keywords: Neoplastic angioendotheliosis ; Malignant lymphoma ; B cell lymphoma ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Frozen cerebral and renal tissue sections of an autopsied “neoplastic angioendotheliosis (NAE)” case were investigated immunohistochemically using monoclonal and heterologous antibodies to lymphocyte, monocyte, endothelial, epithelial and histiocytic antigens. In both tissues, positive stainings for surface immunoglobulin (sIg) μ and ϰ, but not λ, were observed in most of the neoplastic cells. These cells were also positive for other B cell markers (BA-1, Leu-12 and HLA-DR). No distinct staining was observed in the neoplastic cells with antibodies to T lymphocyte (OKT-11 and Leu-1) or monocyte (OKM-1) markers. Posive stainings were observed only in some small round lymphoid cells which were distributed sporadically in and around blood vessels and were considered to be reactive. No positive staining was observed in the neoplastic cells with antibodies to endothelial (factor VIII), epithelial (cytokeratin) or histocytic (lysozyme) antigens. Thus, our NAE case was shown to be of monoclonal B cell lymphoma in nature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690540
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  • 16
    Electronic Resource
    Electronic Resource
    Primary malignant lymphoma of the brain: demonstration of immunoglobulin gene rearrangements in four cases by the Southern blot hybridization technique (1989)
    Springer
    Acta neuropathologica 79 (1989), S. 23-26 
    Details
    ISSN: 1432-0533
    Keywords: Malignant lymphoma ; Brain tumor ; Non-Hodgkin lymphoma ; Immunoglobulin ; Gene rearrangement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using the Southern blot hybridization technique, four cases of the primary malignant lymphomas of the brain, histologically diffuse large cell lymphoma, were examined for the immunoglobulin gene rearrangements. In three lymphomas, the rearrangements were observed in both heavy and light chain genes, providing strong evidence for a B cell lineage of these tumors. On the other hand, in the remaining lymphoma, the rearrangement was observed only in the heavy chain gene. Despite this, immunohistochemical examination revealed positive stainings for heavy and light chain immunoglobulins in tumor cells, suggesting the occurrence of light chain gene rearrangement at the undetectable level. Thus, B lymphocytic differentiation at the gene level was demonstrated in three, or possibly all, of the primary intracerebral malignant lymphomas examined. Since no more than two rearrangements were detected in each immunoglobulin gene, these lymphomas were considered to be monoclonal in nature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00308952
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  • 17
    Electronic Resource
    Electronic Resource
    Increased lipoperoxide value and glutathione peroxidase activity in blood plasma of Type 2 (non-insulin-dependent) diabetic women (1985)
    Springer
    Journal of molecular medicine 63 (1985), S. 765-768 
    Details
    ISSN: 1432-1440
    Keywords: Lipoperoxide ; Glutathione peroxidase ; Catalase ; Cupro-zinc superoxide dismutase ; Type 2 diabetes mellitus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The lipoperoxide values and glutathione peroxidase activity in blood plasma, along with the glutathione peroxidase, catalase and cupro-zinc superoxide dismutase activities in erythrocytes were investigated in 60 women with Type 2 (non-insulin-dependent) diabetes mellitus and in 71 healthy women. The mean lipoperoxide value and the mean plasma glutathione peroxidase activity in the diabetic patients were significantly higher than those in the control subjects (lipoperoxidep〈0.001, plasma glutathione peroxidase activityp〈0.01). The plasma glutathione peroxidase activities did not, however, correlate with the plasma lipoperoxide values. The erythrocyte glutathione peroxidase activity was approximately ten times higher than that of the plasma glutathione peroxidase activity, nor did they correlate with each other. In contrast to the findings of other authors on the activities of the protective enzymes in erythrocytes against oxidative damage, there were no significant differences of erythrocytes glutathione peroxidase, catalase and superoxide dismutase activities between diabetic and control women.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01733829
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  • 18
    Electronic Resource
    Electronic Resource
    Tetrodotoxin-producing bacteria from the blue-ringed octopus Octopus maculosus (1989)
    Springer
    Marine biology 100 (1989), S. 327-332 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several live specimens of the blue-ringed octopus Octopus maculosus were collected from the Philippines in November 1985, and from Japan in February 1986, and the distribution of toxicity, along with toxin composition, in the posterior salivary gland and other soft parts were examined. Tetrodotoxin (TTX: 1400 mouse units g-1) was detected in the posterior salivary gland of a Japanese specimen, while not only the salivary gland but other soft parts were toxic in the Philippine specimens. The Philippine specimens contained TTX and anhydrotetrodotoxin, the Japanese specimen TTX, 4-epitetrodotoxin, and an unknown toxin. The posterior salivary gland, intestine and other parts were excised from the Philippine specimens and examined for bacterial flora. Twenty-two dominant strains were isolated and cultured in a 2xORI medium (Ocean Research Institute, Simidu and Tsukamoto 1985) at 20°C for 20 to 48 h. Cells were harvested by centrifugation, and disrupted by ultrasonication. The toxins were partially purified from the cell lyzate by ultrafiltration and Bio-Gel P-2 column-chromatography. Instrumental analyses disclosed that 16 of the 22 strains produced TTX and/or related substances. Six strains which clearly exhibited TTX productivity were identified as Alteromonas (2 strains), Bacillus (2), Pseudomonas (1) and Vibrio (1), based on biochemical and biological characteristics. Of these, one strain each of Bacillus and Pseudomonas produced TTX at a level detectable by the mouse assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391147
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  • 19
    Electronic Resource
    Electronic Resource
    Corticosteroid effect on early beta-adrenergic down-regulation durign circulatory shock: Hemodynamic study and beta-adrenergic receptor assay (1995)
    Springer
    Intensive care medicine 21 (1995), S. 204-210 
    Details
    ISSN: 1432-1238
    Keywords: Steroids ; Methylprednisolone ; Down-regulation ; Up-regulation ; Catecholamines ; Hemodynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objectives The steroid effect on critically ill patients remains controversial. The aim of this study is to characterize the effect of methylprednisolone on the heart in a beta-adrenergically downregulated condition. Design A prospective hemodynamic study and retrospective receptor assay. Setting Multidisciplinary ICU in a university hospital. Subjects 42 patients who required pulmonary arterial catheters and an additional 4 corpses who were available for study within 3 h of their deaths. Intervention Intravenous methylprednisolone (10 mg/kg). Measurements and results We pursued a hemodynamic study following a glucocorticoid administration. In patients who had undergone a long term (〉72 h) catecholamine treatment, the cardiac index increased. In patients who had undergone a short term (1–72 h) catecholamine treatment and in patients with no record of catecholamine administration, the cardiac index showed no remarkable change. Among the corpuses, who died soon after their arrival, and the patients, who later died in the ward and were available for further study, we measured beta-adrenergic receptor density in the left ventricular myocardium. It was found that receptor density was decreased after long term catecholamine treatment. Methylprednisolone, on the other hand increased the receptor density. Conclusion Methylprednisolone improved the cardiac index, intriguingly, in patients with long term catecholamine treatment in circulatory shock. Myocardial beta-adrenergic receptor also increased in number after the administration of methylprednisolone. However, the hemodynamic improvement caused by methylprednisolone was not observed in patients without beta-adrenergic down-regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01701473
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  • 20
    Electronic Resource
    Electronic Resource
    Pigments of Rubrobacter radiotolerans (1994)
    Springer
    Archives of microbiology 162 (1994), S. 414-421 
    Details
    ISSN: 1432-072X
    Keywords: Carotenoid ; Bacterioruberin ; Red pigment ; Radiotolerance ; Rubrobacter radiotolerans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The highly radioresistant Rubrobacter radiotolerans, contains red pigments. Since the pigments could not be extracted by usual methods, a new method was developed in which the pigments were extracted with organic solvents after addition of 10 N KOH to the intact cells, followed by neutralization. These pigments were also extracted after treatment with achromopeptidase, but not with lysozyme. The extracted pigments separated into two main spots by TLC (48.6% and 22.6%), and were confirmed to be carotenoids by chemical tests. The two major pigments had 13 conjugated double bonds as determined from the main maximum wavelength of the light absorption spectra. Their molecular weights were determined to be 740 and 722 by mass spectrometry. The mass spectra of their TMS-derivatives revealed that they contained four and three tertiary OH groups, respectively. Confirming their identical light and IR spectra, these pigments were determined to be bacterioruberin and monoanhydrobacterioruberin, respectively, the characteristic carotenoids of halophilic bacteria. The existence of these pigments in bacteria other than halobacteria provides interesting new evidence on the distribution of these compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282106
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