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  • 11
    Electronic Resource
    Electronic Resource
    A μ-Oxo-bis[iron(III) porphyrin] Complex (1995)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 51 (1995), S. 2252-2255 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270195005701
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  • 12
    Electronic Resource
    Electronic Resource
    Chitinolytic enzymes: their contribution to basic and applied research (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 468-475 
    Details
    ISSN: 1573-0972
    Keywords: Biocontrol agents ; chitinase/chitosanase ; chitinous waste ; single-cell protein ; ultrastructural studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract After cellulose, chitin is the second most abundant renewable resource available in nature. Marine invertebrates and fungal biomass are the two main sources of chitinous waste, which is commercially exploited. The enzymes involved in chitin degradation have been particularly well studied. Such enzymes have applications in ultrastructural studies, in the preparation of chitooligosaccharides which show anti-tumour activity, as biocontrol agents and in single-cell protein production. Here, the contribution chitin enzymology can make to basic and applied research is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328035
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  • 13
    Electronic Resource
    Electronic Resource
    Purification of α-glucosidae and invertase from bakers' yeast on modified polymeric supports (1999)
    Springer
    Bioseparation 8 (1999), S. 293-306 
    Details
    ISSN: 1573-8272
    Keywords: XAD-16 and NPA-1 ; invertase ; α-glucosidase ; grafting ; ultrasonic irradiation ; adsorption ; desorption ; enrichment factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In the present work Amberlite® XAD-16 and Indion® NPA-1, Polystyrene Divinylbenzene macroreticular spherical resins, have been evaluated quantitatively as supports for the adsorption and isolation of the yeast proteins and the enzymes, invertase and α-glucosidase. Modification of these supports has been carried out by surface grafting using acrylate polymers to reduce the hydrophobicity and nonspecific adsorption of proteins. Good grafting efficiency, in excess of 90%, has been obtained using ultrasonic irradiation for the surface activation of polystyrene resins. XAD-16 has higher adsorption capacities for the total yeast proteins as well as for both the enzymes, α-glucosidase and invertase, than NPA-1 in its respective native and grafted form. Adsorption capacities of XAD-16 and NPA-1 in their respectivenative and grafted forms for α-glucosidase are higher than the capacities for invertase. Nonspecific adsorption of total proteins has been reduced considerably after the grafting of acrylate polymers on hydrophobic supports. At the same time selectivity for the adsorption of both the enzymes has been enhanced on grafted supports. The overall solid-liquid adsorption mass transfer coefficient values (K l a) estimated for adsorption of invertase on XAD are lower than those for α-glucosidase. Native and grafted resins could be regenerated and reused for adsorption of α-glucosidase for two regeneration cycles studied. Storage stability of invertase and α-glucosidase is the same on native and grafted form of XAD-16 and is more than the enzymes in the free form.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008126628635
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  • 14
    Electronic Resource
    Electronic Resource
    Structure of μ-oxo-bis{[N-(2-mercaptoethyl)-N',N'-dimethylethylenediamino]dioxomolybdenum(VI)}, [Mo2O5{(CH3)2NCH2CH2NHCH2CH2S}2] (1985)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 41 (1985), S. 871-873 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270185005807
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  • 15
    Electronic Resource
    Electronic Resource
    Novel iron meso-nitrooctaethylporphine complexes for the catalytic reaction of alkanes with molecular oxygen (1994)
    Springer
    Catalysis letters 24 (1994), S. 79-83 
    Details
    ISSN: 1572-879X
    Keywords: Metallonitroporphines ; oxidation ; isobutane ; propane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Introducing nitro groups into themeso-positions of a metalloporphyrin converts a catalytically inactive complex into a highly active catalyst for the oxidation of alkanes with molecular oxygen. The degree of nitration correlates with both the Fe(III)/Fe(II) reduction potential and the catalytic activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00807377
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  • 16
    Electronic Resource
    Electronic Resource
    Production of β-galactosidase from thermophilic fungus Rhizomucor sp (1997)
    Springer
    Journal of industrial microbiology and biotechnology 19 (1997), S. 239-245 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: β-galactosidase; thermophilic fungus; Rhizomucor; extracellular; solid state fermentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A thermostable β-galactosidase was produced extracellularly by a thermophilic Rhizomucor sp, with maximum enzyme activity (0.21 U mg−1) after 4 days under submerged fermentation condition (SmF). Solid state fermentation (SSF) resulted in a nine-fold increase in enzyme activity (2.04 U mg−1). The temperature range for production of the enzyme was 38–55°C with maximum activity at 45°C. The optimum pH and temperature for the partially purified enzyme was 4.5 and 60°C, respectively. The enzyme retained its original activity on incubation at 60°C up to 1 h. Divalent cations like Co2+, Mn2+, Fe2+ and Zn2+ had strong inhibitory effects on the enzyme activity. The K m and V max for p-nitrophenyl-β- D-galactopyranoside and o-nitrophenyl-β - D-galactopyranoside were 0.39 mM, 0.785 mM and 232.1 mmol min−1 mg−1 respectively. The K m and V max for the natural substrate lactose were 66.66 μM and 0.20 μ mol min−1 mg−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900452
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  • 17
    Electronic Resource
    Electronic Resource
    Effects of taxol and nocodazole on insulin secretion from isolated rat islets of Langerhans (1982)
    Springer
    Bioscience reports 2 (1982), S. 795-801 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Taxol, a promotor of microtubule polymerization, and nocodazole, which induces microtubule depolymerization, used at concentrations known to be specific for these effects in other cell types, were each shown to inhibit glucose-stimulated insulin secretion from isolated rat islets of Langerhans. These findings suggest that the dynamic regulation of microtubule polymerization-depolymerization in pancreatic B ceils may be important for insulin secretion via the microtubule-microfilamentous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01114939
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  • 18
    Electronic Resource
    Electronic Resource
    Concentrations of steroids in the utero-ovarian vein blood, serially collected from the two sides of individual baboons, during the follicullar phase (1986)
    Springer
    Primates 27 (1986), S. 493-506 
    Details
    ISSN: 0032-8332
    Keywords: Baboons ; Ovary ; Steroids ; Gonadotropins ; Follicular phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The purpose of this study was to determine and compare the follicular phase steroid hormone secretion into the utero-ovarian vein by the ovary with a dominant follicle and the contralateral ovary in the same baboon. Serial utero-ovarian vein blood from both sides was collected in 25 baboons by the use of a laparoscope on alternate days, starting on day 1 or 3 of the cycle and continuing through 2 to 3 days post-ovulation. Approximately 3–4 days before the day of expected ovulation, samples were collected at 8-hr intervals. Steroids estradiol (E2) and progesterone (P) were measured in all utero-ovarian vein plasma by radioimmunoassay. In the peripheral plasma, E2, P, LH, and FSH measurements were carried out. Concentrations of steroids were significantly higher on the side of the ovulating ovary by day 5 before ovulation. Individual plots however, indicated that some baboons may establish the dominant side as early as day 11 before ovulation. The preovulatory gonadotropins had a differential effect on the two ovaries. For example, E2 values on the ovulatory side ovary declined after increases in LH/FSH, whereas on the contralateral side these values had increased. Both sides showed increases in the level of P with the increases in LH. The mean interval from E2 peak to LH peak was 24 hrs and LH peak to ovulation was 24 hrs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02381894
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  • 19
    Electronic Resource
    Electronic Resource
    Ovulation pattern in successive cycles in the baboon (1982)
    Springer
    Primates 23 (1982), S. 592-595 
    Details
    ISSN: 0032-8332
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The purpose of this study was to determine if the selection of the side on which ovulation occurs in successive cycles is a random event. Forty-seven baboons were observed for four consecutive cycles and 37 for two to three consecutive cycles. Side of ovulation was determined by laparoscopic examination. Of the 286 cycles studied, 146 cycles (51 %) showed ovulations on the left side and 140 cycles (49 %) on the right. Analysis of the data using two consecutive ovulations in 286 cycles revealed that the selection of the side of ovulation is a random event. However, when four consecutive ovulations observed in 47 baboons were analyzed, it was found that the observed pattern of ovulation was different from that expected from random chance (p〉0.05). There are 16 possible combinations in a sequence of four ovulations. Eight of 47 baboons (17 %) ovulated on the same side for four consecutive cycles, 15 baboons (32 %) ovulated three times on the same side for four ovulations, and 24 baboons (51 %) showed two ovulations on each side. Alternation of ovulation in a sequence of four consecutive ovulations was observed 48 times (25.5 %) and alternation of ovulation in two consecutive cycles was observed 106 times (51 %).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02373973
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