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  • 11
    Electronic Resource
    Electronic Resource
    Molecular cloning of a group of mouse pancreatic islet beta-cell-related genes by random cDNA sequencing (1995)
    Springer
    Diabetologia 38 (1995), S. 381-386 
    Details
    ISSN: 1432-0428
    Keywords: Random cDNA sequencing ; MIN6 ; pancreatic islet beta cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To understand the molecular basis of glucose concentration-responsive insulin synthesis and secretion from pancreatic islet beta cells, a group of pancreatic islet beta-cell-related cDNAs was cloned. A pair of cDNA libraries was constructed from a mouse pancreatic islet beta-cell line of MIN6, which was cultured in either high glucose or low glucose media. By applying a random cDNA sequencing approach, 503 and 395 independent species were obtained from a total of 1,011 and 762 clones in the high glucose and low glucose library, respectively. The unknown genes comprised the majority of about 70% independent clones in both libraries. In Northern blot analysis, 311 (69.4%) of 448 independent clones showed positive signals within 72 h of autoradiographic exposure. Surprisingly, 150 (48.2%) out of 311 positive clones showed positive signals to MIN6 cells, but not to NIH/3T3 fibroblasts. The expression level of three unknown clones were glucose-concentration dependent. Combination of a random cDNA sequencing approach and Northern blot analysis is useful to obtain a large number of novel genes and islet beta-cell-related genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410274
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  • 12
    Electronic Resource
    Electronic Resource
    Detection of mutations in the insulin receptor gene in patients with insulin resistance by analysis of single-stranded conformational polymorphisms (1992)
    Springer
    Diabetologia 35 (1992), S. 261-266 
    Details
    ISSN: 1432-0428
    Keywords: Hyperinsulinaemia ; tyrosine kinase activity ; Type 2 (non-insulin-dependent) diabetes mellitus ; obesity ; screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We analyzed single-stranded conformational poly morphisms to screen for mutations and polymorphisms in the insulin receptor gene in subjects with or without insulin resistance. Using this new technique, we demonstrated the existence of mutations in the insulin receptor gene which we had identified previously. In addition, a new mutation was found in exon 20 of the insulin receptor gene in a patient with moderate insulin resistance associated with morbid obesity, acanthosis nigricans, and polycystic ovary syndrome. The patient was heterozygous for a mutation substituting Leu (CTG) for Pro (CCG) at codon 1178. Pro1178 is a part of a characteristic sequence motif (D1150 F1151 G1152-A1177 P1178 E1179) common to many protein kinases. Analysis of single-stranded conformational polymorphisms was also used to estimate the frequency of a polymorphism at codon 1058. The two codons CAC (1058 His) and CAT (1058 His) both had a prevalence of 50% in 30 Japanese subjects. These data demonstrate that analysis of single-stranded conformational polymorphisms is a simple and sensitive screening method for mutations and polymorphisms in the insulin receptor gene in subjects with or without insulin resistance. Identification of a mutation in the insulin receptor gene in a patient with a moderate degree of insulin resistance associated with morbid obesity suggests that insulin receptor mutations may exist in patients with Type 2 (non-insulin-dependent) diabetes mellitus associated with a moderate degree of insulin resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400927
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  • 13
    Electronic Resource
    Electronic Resource
    Differential expression of glial fibrillary acidic protein in human glioma cell lines (1989)
    Springer
    Acta neuropathologica 78 (1989), S. 9-15 
    Details
    ISSN: 1432-0533
    Keywords: Glioma ; Cell lines ; Glial fibrillary acidic protein ; cDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have obtained a cDNA fragment to human glial fibrillary acidic protein (GFAP) by immunoscreening a λgt11 human brain cDNA library with antibody to bovine GFAP. The highly homologous nucleotide sequence of this clone with that of the mouse GFAP enabled the identification of this cDNA as one encoding GFAP. As this cDNA hybridized with a single major RNA species in Northern blots of RNA from human and mouse brain tissues and gave one or two bands in Southern blots of human genomic DNA, it was considered to be specific for GFAP. Using this cDNA as a probe we investigated the levels of GFAP expression in ten human glioma cell lines. A 3.5-kb GFAP mRNA was detected in five of the ten glioma cell lines, one of which was U-251 MG cell line and the other four were clones derived from the same tumor (CL1, 2, 3, and 4). There was a difference in the amount of GFAP mRNA among U-251 MG and the four clonal cell lines. Quantitative evaluation of this difference by RNA dot blot analysis revealed that the amount of GFAP mRNA expressed in CL3 was about 1/5 and in CL4 about 1/10 the amount expressed in U-251 MG, CL1, and CL2. Semiquantitative Western blot analysis showed that GFAP levels corresponded to the GFAP mRNA levels in these cell lines. By Southern blot analysis of genomic DNA the GFAP gene was similarly detected in all of these cell lines regardless of the level of GFAP expression. Thus, by using a cDNa to human GFAP we have demonstrated the presence of clonal cell lines from human glioma showing different levels of GFAP expression, which may provide a useful basis for further investigations on the regulation of GFAP gene expression in glial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687396
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  • 14
    Electronic Resource
    Electronic Resource
    A case of quadrigeminal hamartoma (1985)
    Springer
    Acta neuropathologica 68 (1985), S. 155-159 
    Details
    ISSN: 1432-0533
    Keywords: Hamartoma ; Heterotopia ; Hydrocephalus ; Aqueduct stenosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A 35-day-old hydrocephalic infant was found by autopsy to have a small nodular mass protruding into the aqueductal channel. The mass, approximately 2.5 mm in size along the neuroaxis and 1.9 mm in transverse plane, subependymally occupied the roof of the aqueduct. This was thought to be the cause of hydrocephalus in this infant. A review of literature on the subject has not shown any other comparable case.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688638
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  • 15
    Electronic Resource
    Electronic Resource
    Structural changes in the hepatopancreas of the rock lobster, Jasus edwardsii (Crustacea: Palinuridae) during development from the puerulus to post-puerulus (1995)
    Springer
    Marine biology 123 (1995), S. 837-844 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There is evidence for non-feeding in pueruli of palinurid lobsters during their long-distance swim to the coast and the succeeding post-settlement phase until they molt to the juveniles. For a better understanding of the nutritional supply during the puerulus stage, structural changes in the hepatopancreas were studied in post-settlement pueruli and first-molt, postpueruli of the rock lobster, Jasus edwardsii, collected on the southeast coast of the North Island, New Zealand between 1989 and 1990. In newly settled (transparent) pueruli, the epithelium of the hepatopancreas is dominated by presumed “young R-(resorptive) cells”, which are devoid of lipid droplets, but show active uptake of material from the hemolymph through pinocytosis at the basal region. In the fully pigmented puerulus, the hepatopancreas is dominated by R-cells containing massive lipid droplets; also present are small numbers of F-(fibrillar) and B-(blister-like) cells. This increase of lipid inclusion in the R-cells corresponds to the reduction in size of the fat bodies as the lobster develops from the transparent to the pigmented puerulus. After the molt to the post-puerulus, the R-cells are compressed, and the lipid droplets almost disappear, while the B-cells increase both in size and number. A mechanism is proposed in the present paper to explain energy storage and utilization during the puerulus and post-puerulus stages, wherein the fat bodies and the lipid inclusions play a vital role.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349128
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  • 16
    Electronic Resource
    Electronic Resource
    Extractable immune complex in soluble substances from psoriatic scales (1984)
    Springer
    Archives of dermatological research 276 (1984), S. 45-51 
    Details
    ISSN: 1432-069X
    Keywords: Psoriasis vulgaris ; Soluble substances of psoriatic scales ; Immune complex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Soluble substances of psoriatic scales (Psoexts) were analysed immunologically to detect immune complex (IC) related to the immune reactions in psoriatic lesions and compared with extracts of scales from a patient with erythroderma due to pityriasis rubra pilaris (PRP-exts) as control. Immunoelectrophoretically IgG, IgA and C3 were detected in Pso-exts, but in PRP-exts only IgG was seen as immunological reactive substances. When analysed by Sephadex G-200 column chromatography, IgG and IgA eluates were found in Pso-exts with a molecular weight of more than 200,000 daltons. By Raji cell assay, the IgG and IgA eluates were shown to have IC characteristics and they were the IC composed of IgG-IgA, which was also confirmed using anti-IgG affinity chromatography. The IC seemed to be binding the epidermal proteins as antigen. Thesefindings suggest that deposits of immunoglobulins and complement in the stratum corneum of psoriatic lesions are not just secondary to the underlying inflammation in the dermis but that the IC detected is related to the immune reactions in psoriatic lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412562
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  • 17
    Electronic Resource
    Electronic Resource
    Ascending and descending components of the medial forebrain bundle in the rat as demonstrated by the horseradish peroxidase-blue reaction (1980)
    Springer
    Experimental brain research 39 (1980), S. 377-388 
    Details
    ISSN: 1432-1106
    Keywords: Medial forebrain bundle ; Ascending components ; Descending components ; HRP ; Sensitive substrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ascending and descending components of the medial forebrain bundle (MFB) were investigated by means of horseradish peroxidase (HRP) with a sensitive substrate. The HRP was injected iontophoretically into the MFB at various levels from the anterior commissure to the posterior hypothalamus. In order to prevent the diffusion of HRP to other brain areas, a double micropipette system was used. The descending components of the MFB are derived from (1) the anterior cingulate area, infra- or prelimbic area, and sulcal cortex, (2) the lateral septal nucleus and diagonal band, (3) the bed nucleus of the stria terminalis, (4) the paraventricular nucleus (5) the substantia innominata, (6) the amygdaloid complex (AM), (7) the ventromedial (VM) and dorsomedial (DM) hypothalamic nuclei, (8) the entopeduncular nucleus and (9) nucleus periventricularis stellatocellularis. The ascending components of the MFB originate in: (1) the medial preoptic nucleus, (2) the nucleus periventricularis stellatocellularis and rotundocellularis, (3) the posterior hypothalamic nucleus, (4) the parafascicular nucleus, (5) the ventral premammillary nucleus, (6) the substantia grisea periventricularis, (7) the lateral habenular nucleus, (8) the VM and DM, (9) the paratenial nucleus, (10) the AM and (11) the arcuate nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239302
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  • 18
    Electronic Resource
    Electronic Resource
    Impaired monocyte function in glycogen storage disease type Ib (1986)
    Springer
    European journal of pediatrics 145 (1986), S. 312-314 
    Details
    ISSN: 1432-1076
    Keywords: Glycogen storage disease type Ib ; Neutrophil ; Monocyte ; Respiratory burst ; Chemotaxis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phagocyte (neutrophil and monocyte) function was evaluated in a boy with glycogen storage disease type Ib. Neutrophils were found to be defective in motility and respiratory burst and monocytes showed a defect in respiratory burst but not in motility. These results suggested that the glucose-6-phosphate transport system plays a role in the function of neutrophils and monocytes and that these two phagocytes are different from each other in their energy metabolism for motility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00439409
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  • 19
    Electronic Resource
    Electronic Resource
    Kawasaki disease associated with idiopathic thrombocytopenic purpura (1989)
    Springer
    European journal of pediatrics 148 (1989), S. 379-379 
    Details
    ISSN: 1432-1076
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00444140
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  • 20
    Electronic Resource
    Electronic Resource
    Kawasaki-like disease in early course of acute monocytic leukaemia (1992)
    Springer
    European journal of pediatrics 151 (1992), S. 177-178 
    Details
    ISSN: 1432-1076
    Keywords: Kawasaki disease ; Acute monocytic leukaemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a rare occurrence of Kawasaki-like disease in an 11-year-old boy with acute monocytic leukaemia (AML). After 1 week of induction therapy with daunorubicin, etoposide, and cytosine arabinoside, the patient sequentially developed persistent fever, lymphadenopathy, conjunctival injection, exanthema, redness of the lips, and desquamation of the hands. Chest roentgenology showed cardiomegaly and an echocardiogram revealed a dilation of the left coronary artery. The patient was treated with high doses of gamma globulin and steroids. All symptoms except the coronary artery dilation improved. The symptoms did not recur on reinstatement of the original antileukaemia drugs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01954378
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