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  • 11
    Electronic Resource
    Electronic Resource
    Analysis of spermatozoa movement using a video imaging technique (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 521-526 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The aim of this paper is to present methods for a fully computerised analysis of spermatocyte movement. The technique were designed for processing pure images of spermatozoa captured from a light microscope. The techniques described allowed for the reduction of background light inhomogeneity and for the correct detection of moving cells and involved densitometric equalisation of background inhomogeneity and implementation of the dynamic threshold, adopted for the recognition of objects. The method for relating cells (found in each frame processed) to movement trajectories used heuristic rules, describing the behaviour of moving cells. This permitted samples containing high numbers of spermatocytes within the observed area to be processed with maximum accuracy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050072
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  • 12
    Electronic Resource
    Electronic Resource
    The assay of acid phosphatase activity by means of a laser method (1970)
    Springer
    Histochemistry and cell biology 24 (1970), S. 245-250 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new method of quantification of the acid phosphatase activity assay by means of laser rays along with spectrography and photometry is described. Calculated ΔS values, representing differences between densities of the spectral bands corresponding to Pb and Ag account for the amount of acP activity. The presented laser method enables a relative evaluation of the acP activity in a given region, yielding results which can be objectively expressed by figures. A special advantage of the presented method is, that it allows to compare the individual acP activities in different regions of the investigated organ, which may be of importance for the quantitative chemoarchitectonic of the brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304194
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  • 13
    Electronic Resource
    Electronic Resource
    Autoradiographic studies on glucose utilization in individual zone of the rat thymus (1988)
    Springer
    Histochemistry and cell biology 88 (1988), S. 571-573 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Wistar rats were injected intraperitoneally with 2-(14C)deoxyglucose and their thymuses were processed for thaw-mount autoradiography after 5, 10 or 35 min. Highest levels of radioactivity were demonstrated in the thymic medulla (5-fold higher than in the cortex). Scanning of autoradiograms for regional differences in grain densities indicated particularly intense glucose utilization in the cortico-medullary zone. Differences in glucose utilization between individual thymic zones seem to reflect differences in cellular composition, i. e., ratio of stroma cells to thymocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00570326
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  • 14
    Electronic Resource
    Electronic Resource
    Preparation of histochemical sections for quantitative determination of acid phosphatase activity by means of laser microanalysis (1971)
    Springer
    Histochemistry and cell biology 26 (1971), S. 74-79 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Based on former experiments dealing with a quantitative assay of acid phosphatase (acP) activity in histological sections by means of a laser microanalysis. The authors present results of investigations concerning optimal conditions needed for this purpose. The investigations were performed on rat liver sections. The realtion between incubation time and depth of acP reaction appearing in tissue blocks was investigated along with the dependence of photometric readings on thickness of histological sections and on incubation times. From results of experiments it appears that optimal conditions for quantitative determination of acP activity using the proposed laser method are provided by sections 30 to 50 μ in thickness which have been incubated in the substrate containing medium for 45 min. Sections thinner than 30 μ are not recommended for quantitative assay with this method because of low accuracy of readings caused by too low amounts of reaction product present there.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307786
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  • 15
    Electronic Resource
    Electronic Resource
    Further investigations concerning the applicability of laser analysis in quantitative histochemistry (1971)
    Springer
    Histochemistry and cell biology 26 (1971), S. 212-217 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Further investigations on the applicability of the laser technique for qantitative purposes in histochemistry were conducted. Special attention has been paid to the relationship between the volume of the crater burnt out in the tissue by laser ray treatment and the photometric readings of the spectral bands. A statistical evalution of the measurements of crater volumes for quantitative histochemistry as well as determination of the error of the former method used and previously described (Kozik et al., 1970b) have been performed. Special agar standards containing known amounts of Pb(NO3)2 and appropriately prepared photographic plates were used. From the results obtained it appeared that the variances calculated for the differences between density of spectral bands for the Pb present in the investigated sample and the density of the respective band for the Ag present in the photographic plate fall in the range of between 14.1 and 19.7%, whereas the error of the method in which the density of spectral bands was related to the crater volumes covered a range from 3.45–7.7%. Based on the discussion of results the authors conclude that both methods of acP assay in histological slices by means of laser microanalysis may be successfully used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305654
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  • 16
    Electronic Resource
    Electronic Resource
    The correlation of individual results in laser microanalysis applied to histological slices (1972)
    Springer
    Histochemistry and cell biology 30 (1972), S. 289-295 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This work is a continuation of investigations concerning the applicability of laser analysis in histochemical techniques (Kozik et al., 1970a, b; Kozik et al., 1971a, b).Agar standards containing known amounts of Pb were subjected to laser microanalysis and optical densities of the resulting Pb spectral bands as well as volumes of the resultant craters were determined.—Based on mathematical considerations and equation has been educed by means of which the experimentally obtained photometric readings may be converted into densities corresponding to mean crater volumes. In that way it is possible to eliminate inconveniences resulting from diversity of individual crater volumes and from eventual differences in the degree of atomic excitation between samples. Using this method, the concentrations of the investigated element in histological slices become expressed in percentages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279777
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  • 17
    Electronic Resource
    Electronic Resource
    Concanavalin A binding sites on the luminal surface of ependymal cells of third ventricle (1978)
    Springer
    Histochemistry and cell biology 58 (1978), S. 139-143 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Detection of the Concanavalin A binding sites within the luminal surface of ependymal cells of the third ventricle has been accomplished using perfusion techniques as a way of exposing the cells to reaction solutions. Parameters for this procedure were established. It was found that electron dense reaction products form a layer ranging from 25–40 nm in thickness on the luminal surface of investigated cells. The microvilli exhibited a slightly thiner layer, the reaction products formed small patches on cilia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489960
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  • 18
    Electronic Resource
    Electronic Resource
    Analysis of spermatozoa movement using a video imaging technique (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 521-526 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The aim of this paper is to present methods for a fully computerised analysis of spermatocyte movement. The technique were designed for processing pure images of spermatozoa captured from a light microscope. The techniques described allowed for the reduction of background light inhomogeneity and for the correct detection of moving cells and involved densitometric equalisation of background inhomogeneity and implementation of the dynamic threshold, adopted for the recognition of objects. The method for relating cells (found in each frame processed) to movement trajectories use heuristic rules, describing the behaviour of moving cells. This permitted samples containing high numbers of spermatocytes within the observed area to be processed with maximum accuracy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02473315
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    Paper (German National Licenses)
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  • 19
    Electronic Resource
    Electronic Resource
    Analysis of colloidal gold methods for labelling proteins (1982)
    Springer
    Histochemistry and cell biology 76 (1982), S. 567-575 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The relationship between unbound and bound proteins prepared during labelling with colloidal gold (Au) was investigated. For this aim, labelled 125I-bovine serum albumin and 125I-rabbit immunoglobulin were employed. The procedures associated with the washing of the Au labelled proteins (i.e. albumin) had a marked influence on the dissociation of the bound ligand. This was most evident when the concentration of albumin that was used for labelling was too high (0.1 or 1.0 mg/ml Au sol). We suggest that purification of labelled proteins be conducted shortly before use so as to avoid a significant amount of dissociation during the time when the solution is coming to equilibrium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489911
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  • 20
    Electronic Resource
    Electronic Resource
    Distribution of Lyt antigens on the surface of thymocytes associated with thymic macrophages and dendritic cells (1988)
    Springer
    Histochemistry and cell biology 88 (1988), S. 553-556 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thymocyte subpopulations that are associated with macrophages and dendritic cells of the thymus in vivo were isolated from the thymuses of C57Bl/6 mice, and their Lyt phenotypes were analyzed. Electron-microscopic examination of immunogold-labeled cells revealed that the thymic complexes formed by macrophages mainly contained Lyt-2-positive thymocytes, while Lyt-1-positive thymocytes were more frequently associated with dendritic cells. The characteristic distributions of Lyt antigens on the surface of thymocytes in regions of reciprocal contact with macrophages (Lyt-2-positive cells) and dendritic cells (Lyt-1-positive cells) suggest that these antigens play a role in specific interactions between thymocytes and stroma cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00570323
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