ISSN:
1434-4726
Schlagwort(e):
Selenium
;
Vitamin A
;
Butylated hydroxytoluene
;
Chemoprevention
;
Human tongue cancer cells in vitro
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract The effects of vitamin A, selenium and butylated hydroxytoluene (BHT) on the growth of a human tongue cancer cell line were examined in monolayer cell cultures. A colony-forming assay showed a 50% reduction in the survival rate of the cell line at a concentration of 2.6 μg/ml selenium, 60 I μg/ml vitamin A and/or 38 μg/ml BHT. Relatively low concentrations of selenium markedly inhibited glucose consumption. Flow cytometric analysis with both fluorescein-isothiocyanate-labelled bromodeoxyuridine monoclonal antibody and propidium iodide demonstrated an increase in S-phase fractions 1 day after the addition of selenium, increased GO/G1 phase fractions in the presence of vitamin A and increased G2M phase fractions when BHT was added. These results suggest that these compounds inhibit DNA synthesis of in vitro human tongue cancer cells by different mechanisms.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02114762
Permalink
