Digitale Medien
Oxford, UK
:
Blackwell Publishing Ltd
Clinical & experimental allergy
11 (1981), S. 0
ISSN:
1365-2222
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Total IgE was determined in 107 sera using a novel, automated, non-isotopic immunoassay called PACIA (particle-counting immunoassay) based on agglutination of anti-IgE coated latex particles by IgE. The IgE values ranged from 10 to 50 000 iu/ml and were compared with results obtained by a conventional radioimmunoassay (RIA) which used a fast double antibody separation technique the coefficient of correlation was 0.985 and the regression line r = 0-82 x+ 130.00.PACIA had several advantages over the RIA technique: using a sampling rate of 50.hr. results were obtained in 35 min compared to 16-20 hr. no labelled IgE was required and the separation step, which relied on measuring the number of non-agglutinated particles by an optical cell counter, was fully automated. The threshold of sensitivity was 10 iu/ml and the maximal coefficient of variation for between assay precision was 12-9%.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1365-2222.1981.tb01619.x
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