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A Mutational Analysis of DNA Binding and Cleavage by the Homing Endonuclease PI-SceI (2000)

Wende, W. ; Schöttler, S. ; Grindl, W. ; [weitere]

Springer

Molecular biology 34 (2000), S. 902-912

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ISSN: 1608-3245

Schlagwort(e): intein ; protein–nucleic acid interaction ; DNA binding ; DNA bending ; phosphodiester bond cleavage ; mechanism ; site-directed mutagenesis

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract We have carried out an extensive mutational analysis of PI-SceI, the best studied intein-like homing endonuclease of the LAGLIDADG family, to find out which amino acid residues are involved in substrate binding and processing. Our analysis was focused on domain I, in which two regions were shown to be in contact with DNA, and on domain II, in which the amino acid residues making up catalytic centers I and II were identified and their role in catalysis investigated. As a result of our comprehensive mutational analysis a model is presented for DNA binding and cleavage by PI-SceI.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1026636011589

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Digitale Medien

Some observations on the WATR method for water suppression at 80 MHz (1987)

Dickinson, Noel A. ; Lythgoe, Ronald E. ; Waigh, Roger D.

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 25 (1987), S. 996-997

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ISSN: 0749-1581

Schlagwort(e): 1H NMR ; WATR ; Water suppression ; Ammonium chloride ; Guanidinium chloride ; Buffer salts ; Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: The WATR method for water suppression in 1H NMR spectroscopy has been evaluated at 80 MHz, using both ammonium and guanidinium chloride, with and without the addition of buffer salts. The pH of maximum effect has been found to depend on field strength and to be markedly affected by buffer salts, particularly phosphates. Provided that the pH is carefully controlled the method is simple and effective.

Zusätzliches Material: 5 Ill.