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Digitale Medien

Incorporation of dispersion into the theory of electrophoresis of DNA and its complexes (1998)

Cann, John R.

Weinheim : Wiley-Blackwell

Electrophoresis 19 (1998), S. 1336-1340

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ISSN: 0173-0835

Schlagwort(e): Computer simulations ; DNA ; Dispersion ; Asymmetry ; Mobility-shift assay ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: Incorporation of the dispersion coefficient into the theory of the mobility-shift assay for DNA-protein complexes was highly successful largely due to increased mathematical rigor. A model simulating electrophoretic migration of DNA across the phase boundary between the initial zone of macromolecule and the gel lane predicts the peak asymmetry observed experimentally. It also predicts that, under the agency of the dispersion coefficient, the peak will become progressively more symmetrical during migration along the gel lane.

Zusätzliches Material: 2 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150190822

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