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  • 1
    Digitale Medien
    Digitale Medien
    Characterization of bacterial diversity in Pulque, a traditional Mexican alcoholic fermented beverage, as determined by 16S rDNA analysis (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 235 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The bacterial diversity in pulque, a traditional Mexican alcoholic fermented beverage, was studied in 16S rDNA clone libraries from three pulque samples. Sequenced clones identified as Lactobacillus acidophilus, Lactobacillus strain ASF360, L. kefir, L. acetotolerans, L. hilgardii, L. plantarum, Leuconostoc pseudomesenteroides, Microbacterium arborescens, Flavobacterium johnsoniae, Acetobacter pomorium, Gluconobacter oxydans, and Hafnia alvei, were detected for the first time in pulque. Identity of 16S rDNA sequenced clones showed that bacterial diversity present among pulque samples is dominated by Lactobacillus species (80.97%). Seventy-eight clones exhibited less than 95% of relatedness to NCBI database sequences, which may indicate the presence of new species in pulque samples.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09599.x
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  • 2
    Digitale Medien
    Digitale Medien
    Characterization of the 5′ subtilisin (aprE) regulatory region from Bacillus subtilis (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 183 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The aprE gene of Bacillus subtilis encodes the major serine alkaline protease known as subtilisin. It is expressed during the transition state and transcribed by the σA form of the RNA polymerase (RNAP). In this work, we characterized the regulatory region of the aprE gene (rraprE) from B. subtilis. By computer analysis and site-directed mutagenesis, we localized the aprE promoter sequence 7 bp upstream from its transcription initiation site (TIS). We also characterized the static curvature properties of the rraprE DNA and found two different areas of DNA bending, within the first 400 bp upstream of its TIS. We postulate that these particular curved DNA regions could play a role in the interaction with some regulatory proteins and discuss possible implications related to aprE transcription regulation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb08926.x
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  • 3
    Digitale Medien
    Digitale Medien
    Carbon regulation and the role in nature of the Escherichia coli penicillin acylase (pac) gene (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Quantitative analysis of specific pac mRNA and a lacZ fusion to the 5’-terminal region of the pac gene demonstrated that both phenylacetic acid induction and catabolite repression by glucose are involved, at the transcriptional level, in the regulation of the pac gene. The studies presented here suggest that this regulation is also present in Escherichia coli transformed strains in which the pac gene was not originally present. Analysis of the nucleotide sequence of the 5′-terminal region of this gene, with a statistical algorithm, confirms that the putative promoter previously proposed by our group is the most feasible within this region. We demonstrate that penicillin acylase activity can confer on E. coli the ability to use penicillin G as a metabolic substrate, by detaching the phenylacetic group which can be used as a carbon source. Based on these data, the regulation properties of the pac gene studied in this work, and the specificity profile of the penicillin acylase enzyme we suggest a role for it in E. coli as a scavenger enzyme for phenylacetylated compounds.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb01391.x
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  • 4
    Digitale Medien
    Digitale Medien
    gltF, a member of the gltBDF operon of Escherichia coli, is involved in nitrogen-regulated gene expression (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: We report here the construction and analysis of insertional mutations in each of the three genes of the gltBDF operon and the nucleotide sequence of the region downstream from gltD. Two open reading frames were identified, the first of which corresponds to gltF. The gltB and gltD genes code for the large and small subunits, respectively, of the enzyme glutamate synthase (GOGAT). gltF codes for a protein, with a molecular mass of 26350Da, which is required for Ntr induction. Histidase synthesis was determined as a measure of Ntr function. First, insertions in gltB, gltD or gltF all prevent Ntr induction. Second, complementation analysis indicates that high-level expression of both the gltD and gltF genes is required for the induction of the Ntr enzymes under nitrogen-limiting conditions, indicating that the phenotype of the gltB insertion probably results from polarity on gltD and gltF. Third, glutamate-dependent repression of the glt operon appears to be mediated by the product of the gltF gene. Thus, the gltBDF operon of Escherichia coli is involved in induction of the so-called Ntr enzymes in response to nitrogen deprivation, as well as in glutamate biosynthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb01450.x
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  • 5
    Digitale Medien
    Digitale Medien
    Biotechnology in Mexico: Planning for the future (1997)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 15 (1997), S. 742-743 
    Details
    ISSN: 1546-1696
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: [Auszug] As the fourth richest region in the world in terms of biodiversity, Mexico is endowed with animals, plants, insects, and microbes that cannot be found anywhere else on the planet, and that, unlike petroleum (whose exploitation and commercialization have been fundamental to economic development), ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/nbt0897-742
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  • 6
    Digitale Medien
    Digitale Medien
    Pathway engineering for the production of aromatic compounds in Escherichia coli (1996)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 14 (1996), S. 620-623 
    Details
    ISSN: 1546-1696
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: [Auszug] Glucose is the preferred substrate for certain fermentation processes. During its internalization and concomitant formation of glucose-6-phosphate through the glucose phosphotransferase system (PTS), one molecule of phosphoenolpyruvate (PEP) is consumed. Together with erythrose 4-phosphate (E4P), ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/nbt0596-620
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  • 7
    Digitale Medien
    Digitale Medien
    Technology Transfer: Changing university roles in the century of biotechnology (1998)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 16 (1998), S. 598-599 
    Details
    ISSN: 1546-1696
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: [Auszug] Biotechnology has thrown a powerful spotlight on the complex and changing nature of society's expectations for universities. On the one hand, society wants universities to serve as the repository of knowledge, pass along knowledge to students, and promote discovery that extends the frontiers of ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/nbt0798-598
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  • 8
    Digitale Medien
    Digitale Medien
    A new expression vector for the production of fused proteins in Escherichia coli (1986)
    Springer
    Applied microbiology and biotechnology 25 (1986), S. 267-271 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary The construction of a new expression vector for fused proteins production in Escherichia coli is reported. This new vehicle uses the trp promoter-operator control region for the high level expression of a DNA fragment that codes for the amino terminal fragment of the cI λ repressor protein. This truncated polypeptide is accumulated as inclusion bodies that are easily purified. To probe the benefits of the system, synthetic DNA that codes for the human insulin B chain, was cloned at the end of the DNA coding region for the cI truncated peptide. The hybrid peptide thus produced after induction, allowed an easy and reproducible purification of active insulin B chain.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00253661
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  • 9
    Digitale Medien
    Digitale Medien
    New insights on the comma-less theory (1992)
    Springer
    Origins of life and evolution of the biospheres 21 (1992), S. 251-254 
    Details
    ISSN: 1573-0875
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie
    Notizen: Abstract The comma-less hypothesis represents a theoretical effort to describe one of the steps in the early evolution of the translation apparatus. This hypothesis emphasizes the advantages that a RNY coding pattern would have provided in a primitive RNA adaptor-catalyst system. This theory has been debated for years, both in conceptual and statistical terms, and no consensus about its validity has been ascertained. In this work, a statistical model refuting this theory was reconsidered. This new approach eliminates the bias due to the absence of stop codons in the open reading frame, and to the amino acid composition of bacterial genes. The results obtained support the biological significance of the RNY coding pattern.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01809860
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  • 10
    Digitale Medien
    Digitale Medien
    Stimulation of glucose catabolism through the pentose pathway by the absence of the two pyruvate kinase isoenzymes in Escherichia coli (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 58 (1998), S. 292-295 
    Details
    ISSN: 0006-3592
    Schlagwort(e): metabolic engineering ; carbon metabolism ; Escherichia coli mutants ; microbial growth ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Escherichia coli strains devoid of one or both of the two pyruvate kinase isoenzymes (PKA and PKF), were grown on minimal media in batch fermentations. The strain lacking both PKs showed a 28% decrease on its specific growth rate when compared to the wild type. However, protein and CO2 yields did not change. Using radioactive 1-C14 glucose and collecting the CO2 produced by the cultures, it was found that the mutant lacking both pyruvate kinases, metabolized glucose mainly through the pentose pathway (PP). The increased participation of the PP in glucose metabolism in this strain, was also reflected on the levels of the glucose-6-phosphate and 6-phosphogluconate dehydrogenases.© 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:292-295, 1998.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
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