ISSN:
1440-1681
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
1. The aim of this study was to investigate protein kinase C (PKC) gene expression in spontaneously hypertensive rats (SHR).2. Using the PKC oligodeoxyribonucleotide probes (γ, ε), we detected PKC isoforms gene expression in the heart and brain of 4 and 20 week old SHR with those of age-matched Wistar-Kyoto (WKY) rats by northern blot analysis.3. In the cerebral cortex, there were significantly increased levels of expression of the Ca2+-dependent isoform PKC-γ in 4 and 20 weeks SHR compared with that of WKY, while Ca2+-independent isoform PKC-ε did not differ between SHR and WKY.4. In ventricular myocytes, there was a significant expression of the Ca2+-independent isoform PKC-ε in 4 and 20 week old SHR compared with that of WKY, while Ca2+-dependent isoform PKC-γ could not be detected in the same extracts of SHR or WKY.5. We conclude that both of the Ca2+-dependent and Ca2+-independent PKC could be involved in the pathogenesis of SHR. Ca2+-dependent PKC-γ may be mainly involved in the modulation of blood pressure in the level of the central nervous system, while Ca2+-independent PKC-ε Could be related to the genetic myocardial hypertrophy.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1440-1681.1995.tb02884.x
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