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Digitale Medien

The effect of cytochalasin B on the rate of growth and ultrastructure of wheat coleoptiles and maize roots (1979)

Pope, D. G. ; Thorpe, J. R. ; Al-Azzawi, M. J. ; [weitere]

Springer

Planta 144 (1979), S. 373-383

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ISSN: 1432-2048

Schlagwort(e): Auxin ; Cell elongation ; Cytochalasin B ; Dictyosomes ; Secretion ; Triticum ; Zea

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Cytochalasin B (CB) inhibits the elongation growth of maize roots, and that of wheat coleoptile segments incubated in indolyl-3-acetic acid, by over 30% after a lag period of about 60 min. This long lag is not due to poor tissue penetration by the inhibitor, but seems to reflect a property of the process inhibited by CB. The only visible ultrastructural change accompanying growth inhibition is the accumulation of secretory vesicles in the vicinity of dictyosomes, which occurs between 90 and 300 min. However, a massive accumulation of vesicles is seen after 120 min in root cap cells which possess very active dictyosomes. The results indicate that CB does not inhibit elongation growth by interfering with cytoplasmic streaming. Instead, they indicate that the drug acts to inhibit the secretion of cell wall components at some stage after vesicle production, but prior to their transport.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00391581

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Digitale Medien

The role of the epidermis in auxin-induced and fusicoccin-induced elongation of Pisum sativum stem segments (1980)

Brummell, David A. ; Hall, J. L.

Springer

Planta 150 (1980), S. 371-379

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ISSN: 1432-2048

Schlagwort(e): Auxin ; Cell elongation ; Epidermis peeling ; Fusicoccin ; Pisum

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The effects of peeling and wounding on the indole-3-acetic acid (IAA) and fusicoccin (FC) growth response of etiolated Pisum sativum L. cv. Alaska stem tissue were examined. Over a 5 h growth period, peeling was found to virtually eliminate the IAA response, but about 30% of the FC response remained. In contrast, unpeeled segments wounded with six vertical slits exhibited significant responses to both IAA and FC, indicating that peeling does not act by damaging the tissue. Microscopy showed that the epidermis was removed intact and that the underlying tissue was essentially undamaged. Neither the addition of 2% sucrose to the incubation medium nor the use of a range of IAA concentrations down to 10-8 M restored IAA-induced growth in peeled segments, suggesting that lack of osmotic solutes and supra-optimal uptake of IAA were not important factors over this time period. It is concluded that, although the possibility remains that peeling merely allows leakage of hydrogen ions into the medium, it seems more likely that peeling off the epidermis removes the auxin responsive tissue.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00390172

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Digitale Medien

Dual capillary column supercritical fluid chromatography (1990)

Raynor, M. W. ; Moulder, R. ; Davies, I. L. ; [weitere]

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 13 (1990), S. 22-26

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ISSN: 0935-6304

Schlagwort(e): Supercritical fluid chromatography ; Dual capillary columns ; Polycyclic aromatic hydrocarbons ; Hop and beer bitter acids ; Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: A chromatograph equipped with one injection valve, two capillary columns with different selectivities, two flame ionization detectors and two electrometers is used to perform simultaneous dual capillary supercritical fluid chromatography. Two sets of retention times and indices are obtained for a mixture of polycyclic aromatic hydrocarbons, from one injection, enabling positive identification. The system is also used to rapidly select a stationary phase for separating hop and beer bittering acids.

Zusätzliches Material: 8 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jhrc.1240130106

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Digitale Medien

An application of the INEPT pulse sequence to the NMR assignment of halogenated marine natural products (1983)

Brownlee, R. T. C. ; Hall, J. G. ; Reiss, J. A.

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 21 (1983), S. 544-547

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ISSN: 0030-4921

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: The routine use of the INEPT pulse sequence is shown to be the method of choice for the assignment of multiplicity from 13C NMR spectra where no prior information about the 1J(CH) coupling constants is available, or where these are expected to vary over a large range. This technique is illustrated using spectra obtained from polyhalogenated monoterpenes isolated from the red alga Plocamium angustum, which have 1J(CH) values varying from 127 to 203 Hz.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/omr.1270210905

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