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  • Southern blot  (1)
  • clarity  (1)
  • 1
    ISSN: 1573-5117
    Keywords: macrophytes ; lakes ; clarity ; light attenuation ; PAR ; New Zealand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seasonal variation in water clarity, as indicated by the attenuation coefficient for photosynthetically active radiation, K d (m-1), was determined by monthly measurements for a year in 9 North Island, New Zealand lakes. K d varied by a factor of 2 to 3 in 8 of the lakes, and a factor of 5 in one. Annual mean K d (symbol% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaam4saaaa!36BD!\[K\] d) varied by a factor of approximately 15 between lakes. The maximum depth of water colonized by macrophytes (z c)was also determined. Values of z c were in the range 1.5–12.5 m. The relationship z c =4.34/% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaam4saaaa!36BD!\[K\]d accounted for most (93 percent) of the variability in z c , indicating that average annual clarity was probably a useful predictor of z c in lakes in this region. The values of z c in these North Island lakes were generally greater than values calculated using previously published empirical relationships derived for northern hemisphere groups of lakes. The extent to which these relationships underestimated z c in the North Island lakes was broadly related to latitude. Estimated average irradiance at z c in each lake was similar to compensation point irradiances reported previously for freshwater macrophytes.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-4927
    Keywords: Rh-related gene ; R-C-E-F system ; molecular genetics ; Southern blot ; phylogeny ; sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract As the chimpanzee R-C-E-F blood group system appears to be the chimpanzee counterpart of the human Rhesus (RH) system, we have tried to determine whether chimpanzee Rh-like genes encode R-C-E-F-related proteins. Chimpanzee genomic DNA, digested by any of eight endonucleases and hybridized with three Rh exon-specific probes, exhibits a high degree of polymorphism. Analysis of DNA from unrelated individuals of different R-C-E-F types revealed that the presence of some restriction fragments is correlated with particular R-C-E-F types. The cosegregation of these fragments with R-C-E-F haplotypes was confirmed by family studies. Oligonucleotides complementary to regions flanking human exons were used as PCR primers on chimpanzee DNA; the resulting amplified fragments were identical in size to their human counterparts. Moreover, the nucleotide sequences of the fragments present a high degree of similarity to the corresponding human regions.
    Type of Medium: Electronic Resource
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