ZIB

Treffer pro Seite

Treffer 1 - 1 | 1 Treffer

Sortierung

Digitale Medien

Measurement of intracellular LDH activity in 96-well cultures: A rapid and automated assay for cytotoxicity studies (1991)

Ponsoda, Xavier ; Jover, Ramiro ; Castell, José Vicente ; [weitere]

Springer

Methods in cell science 13 (1991), S. 21-24

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1573-0603

Schlagwort(e): intracellular lactate dehydrogenase ; tetrazolium salt ; micromethod

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary The present paper describes a rapid, easy, sensitive, and automated spectrophotometric assay for intracellular lactate dehydrogenase (LDH) measurement in 96-well plates of adherent cells for cytotoxicity studies. The procedure involves “in situ” homogenization of cells, followed by measurement of LDH activity with a colorimetric method based on the reduction of a tetrazolium salt to a violet formazan by the NADH formed by LDH. Color intensity can be measured in conventional ELISA readers, and the data can be fed to an “on line” computer for rapid processing. The color absorbance measured is time- and enzyme-concentration dependent. LDH activity measured with this micromethod is coincident with that measured in larger culture plates after individual homogenization and conventional LDH measurement. The advantages of this method are the smaller number of cells required, easy automation, drastic reduction of time for processing individual wells, and the possibility of examining multiple variables in the same experiment.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02388199

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Treffer 1 - 1 | 1 Treffer