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  • 1
    Digitale Medien
    Digitale Medien
    An 83-kDa embryonic-type nuclear antigen is detected within the germinal vesicles of oocytes of the ascidianHalocynthia roretzi (1990)
    Springer
    Development genes and evolution 199 (1990), S. 207-211 
    Details
    ISSN: 1432-041X
    Schlagwort(e): 83-kDa nuclear antigen ; Germinal vesicles ; Ascidian embryogenesis ; Monoclonal antibody
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Monoclonal antibodies were raised against germinal vesicles which were isolated from fully grown oocytes of the ascidianHalocynthia roretzi. Immunoblot analyses revealed that one of the antibodies, designated Hgv-2, recognized a single band with a molecular weight of about 83 kDa. The antibody, visualized by indirect immunohistochemistry, reacted only with the germinal vesicles of oocytes and did not react with test cells, follicle cells, and other somatic cells of the gonad. During embryogenesis the antigenicity was found in interphase nuclei of all embryonic cells. The antibody did not react with chromosomes or the mitotic apparatus. The antigenicity was retained by interphase nuclei of larval cells, but it disappeared from nuclei of juveniles about 7 days after metamorphosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01682079
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  • 2
    Digitale Medien
    Digitale Medien
    Expression of genes for two C-type lectins during budding of the ascidian Polyandrocarpa misakiensis (1995)
    Springer
    Development genes and evolution 204 (1995), S. 406-411 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Ascidian ; Budding ; C-type lectin ; Molecular cloning ; Gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Two closely related cDNA fragments, named pTC14-1 and pTC14-2, encoding C-type lectins were cloned from the budding ascidian Polyandrocarpa misakiensis by means of the polymerase chain reaction. The amino acid sequence deduced from pTC14-1 was identical to that of a 14-kDa calcium-dependent galactose-binding lectin, TC-14, that had been purified from this species. Between the two clones, nucleotide sequence similarity was 90%, whilst that of the deduced amino acid sequences was 82%. The cDNA inserts of these clones hybridized weakly with each other. Antisense RNA probes prepared from these clones gave intense hybridization signals on Northern blots of the W strain, but very weak signals on those of the other strains. Therefore, both clones were suggested to originate from the W strain, but from two separate genes, since the base substitution was scattered throughout the entire translated region. The amount of TC14-1 mRNA increased during bud development, and peaked at 36 h after separation of the bud from the parental body wall. At this stage, extracellular matrix containing TC-14 lectin developed in the mesenchymal space around the morphogenetic region of the bud. There was much less TC14-2, than TC14-1 mRNA at every stage of bud development. TC14-1 and TC14-2 mRNAs were detected on Northern blots of RNAs from adults and growing buds, suggesting that these genes can be used as the earliest markers of budding in this species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00360486
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  • 3
    Digitale Medien
    Digitale Medien
    Specific expression of myosin heavy chain gene in muscle lineage cells of the ascidian embryo (1990)
    Springer
    Development genes and evolution 199 (1990), S. 307-313 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Specific gene expression ; Myosin heavy chain ; Muscle lineage cells ; Ascidian embryos
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In ascidians the lineage of embryonic precursor cells is well documented. In this study, we investigated temporal and spatial expression of myosin heavy chain (MHC) gene during embryogenesis of the ascidianHalocynthia roretzi. When the occurrence of MHC transcripts was examined by Northern blot hybridization and in situ hybridization with specific cDNA and antisense RNA probes, transcripts were undetectable in fertilized eggs and cleavage-stage embryos. This suggests that the maternal message for MHC is not directly associated with the synthesis of MHC protein in ascidian embryos. MHC transcripts were initially observed at the gastrula stage. In situ hybridization of whole-mount preparations demonstrated that the transcripts first appeared in nuclei of primary-lineage muscle cells of the early-to-middle gastrula and accumulated rapidly as development progressed. The occurrence of MHC transcripts was restricted to differentiating muscle cells. No hybridization signal was detected in mesenchyme cells which are thought to form adult body-wall muscle. After metamorphosis the amount of MHC transcripts decreased; they became undetectable in juveniles about 10 days after metamorphosis, suggesting an intermission of MHC gene activity prior to the formation of adult bodywall muscle. Thus the expression of MHC gene in ascidian embryos was strictly regulated in both spatial and temporal orders and occurred only in muscle lineage cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01709509
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