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Digitale Medien

Cytoskeletal protein synthesis and organization in cultured mouse osteoblastic cells Effects of cell density

Lomri, A. ; Marie, P.J. ; Escurat, M. ; [weitere]

Amsterdam : Elsevier

FEBS Letters 222 (1987), S. 311-316

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ISSN: 0014-5793

Schlagwort(e): (Mouse bone cell) ; Actin ; Cell density ; Cytoskeleton ; Tubulin ; Vimentin

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Chemie und Pharmazie , Physik

Materialart: Digitale Medien

URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(87)80392-7

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Digitale Medien

Effect of parathyroid hormone and forskolin on cytoskeletal protein synthesis in cultured mouse osteoblastic cells

Lomri, A. ; Marie, P.J.

Amsterdam : Elsevier

Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 970 (1988), S. 333-342

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ISSN: 0167-4889

Schlagwort(e): (Murine endosteal osteoblasts) ; Actin ; Cytoskeleton ; Forskolin ; Parathyroid hormone ; Polymerization ; cyclic AMP

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Chemie und Pharmazie , Medizin , Physik

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/0167-4889(88)90133-4

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Digitale Medien

Distinct effects of calcium- and cyclic AMP-enhancing factors on cytoskeletal synthesis and assembly in mouse osteoblastic cells

Lomri, A. ; Marie, P.J.

Amsterdam : Elsevier

Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 1052 (1990), S. 179-186

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ISSN: 0167-4889

Schlagwort(e): (Mouse osteoblast cells) ; Actin synthesis ; Calcium, intracellular ; Cytoskeletal synthesis ; Tubulin synthesis ; cAMP

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Chemie und Pharmazie , Medizin , Physik

Materialart: Digitale Medien

URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4889(90)90074-N

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Digitale Medien

Genomic Insertion of the SV-40 Large T Oncogene in Normal Adult Human Trabecular Osteoblastic Cells Induces Cell Growth Without Loss of the Differentiated Phenotype (1999)

Lomri, A. ; Fromigué, O. ; Hott, M. ; [weitere]

Springer

Calcified tissue international 64 (1999), S. 394-401

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ISSN: 1432-0827

Schlagwort(e): Key words: Adult — Human — Immortalization — Trabecular osteoblasts — Mineralization.

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Physik

Notizen: Abstract. In the present study, we established a new adult human trabecular osteoblastic (AHTO) cell line, immortalized by SV-40 Large T (LT) oncogene. From seven proliferative colonies identified, we selected clone 7 with high alkaline phosphatase (ALP) activity for further analysis. AHTO−7 cells were able to grow for at least 8 months and 25 passages, with a doubling time of about 22 hours. Immunocytochemistry staining and RT-PCR analysis indicated that the extended life-span of AHTO−7 cells results in genomic insertion of SV-40 LT oncogene. The cells responded to PTH and PGE2 in terms of cAMP accumulation. The time course study, in the presence of 10−8 M vitamin D3 (vit D3) showed a marked increase (fourfold) in ALP activity with a peak at day 3. Furthermore, in the presence of ascorbic acid (50 μg/ml) and inorganic phosphate (3 mM), AHTO−7 cells produced abundant calcified extracellular matrix, as examined by the von Kossa staining after 2 weeks of culture. Molecular analysis of mRNAs for phenotypic osteoblast markers at day 15 showed the expression of ALP, osteocalcin (OC), and collagen type I (Col I) mRNAs constitutively. Col I expression was inhibited by vit D3 and dexamethasone treatment. In contrast, treatment with vit D3 induced a marked increase of ALP and OC transcripts. Therefore, the immortalized AHTO−7 cells express osteoblast markers that are induced by calciotropic hormones, and constitute a suitable model for identifying specific osteoblastic genes and their regulation during human osteoblast differentiation.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/PL00005821

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Digitale Medien

Bone morphogenetic protein-2 and transforming growth factor-β2 interact to modulate human bone marrow stromal cell proliferation and differentiation (1998)

Fromigué, O. ; Marie, P.J. ; Lomri, A.

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 68 (1998), S. 411-426

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ISSN: 0730-2312

Schlagwort(e): bone marrow stroma ; human ; differentiation ; TGF-β ; BMP-2 ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: Osteoprogenitor cells in the human bone marrow stroma can be induced to differentiate into osteoblasts under stimulation with hormonal and local factors. We previously showed that human bone marrow stromal (HBMS) cells respond to dexamethasone and vitamin D by expressing several osteoblastic markers. In this study, we investigated the effects and interactions of local factors (BMP-2 and TGF-β2) on HBMS cell proliferation and differentiation in short-term and long-term cultures. We found that rhTGF-β2 increased DNA content and stimulated type I collagen synthesis, but inhibited ALP activity and mRNA levels, osteocalcin production, and mineralization of the matrix formed by HBMS cells. In contrast, rhBMP-2 increased ALP activity and mRNA levels, osteocalcin levels and calcium deposition in the extracellular matrix without affecting type I collagen synthesis and mRNA levels, showing that rhBMP-2 and rhTGF-β2 regulate differentially HBMS cells. Co-treatment with rhBMP-2 and rhTGF-β2 led to intermediate effects on HBMS cell proliferation and differentiation markers. rhTGF-β2 attenuated the stimulatory effect of rhBMP-2 on osteocalcin levels, and ALP activity and mRNA levels, whereas rhBMP-2 reduced the rhTGF-β2-enhanced DNA synthesis and type I collagen synthesis. We also investigated the effects of sequential treatments with rhBMP-2 and rhTGF-β2 on HBMS cell differentiation in long-term culture. A transient (9 days) treatment with rhBMP-2 abolished the rhTGF-β2 response of HBMS cells on ALP activity. In contrast, a transient (10 days) treatment with rhTGF-β2 did not influence the subsequent rhBMP-2 action on HBMS cell differentiation. The data show that TGF-β2 acts by increasing HBMS cell proliferation and type I collagen synthesis whereas BMP-2 acts by promoting HBMS cell differentiation. These observations suggest that TGF-β2 and BMP-2 may act in a sequential manner at different stages to promote human bone marrow stromal cell differentiation towards the osteoblast phenotype. J. Cell. Biochem. 68:411-426, 1998. © 1998 Wiley-Liss, Inc.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

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