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  • 1
    Digitale Medien
    Digitale Medien
    A Simplified Overlay Plaque Technic for Evaluating Responses of Small Free-Living Amebae in Grassland Soils (1975)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 22 (1975), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: SYNOPSIS. The responses of amebae and bacteria in a grassland soil were investigated by an overlay plaque technic developed in this laboratory. This procedure, using Aerobacter aerogenes as the food source, allowed convenient assay of significant changes in ameba populations which resulted from additions of nutrient and water. In comparison with controls, when water was added an initial increase occurred in bacterial counts followed by an increase in the numbers of amebae. Upon addition of glucose, ameba populations increased initially and then decreased with time, while populations of bacteria remained constant. The addition of hay resulted in significant increases in populations of bacteria and amebae. Plaque appearance on enumeration plates was most rapid with inocula from nutrient-treated soils.Predominant amebae recovered by this technic were species of Acanthamoeba and Hartmannella. They were estimated to be present in untreated soils at 3.2 × 103/gram. Ameba feeding experiments were used to evaluate the possible suitability of other bacteria as food. The results indicated that nonpigmented laboratory strains of bacteria were preferred, while pigmented grassland isolates were more rapidly utilized.Small soil amebae appear to be sensitive to minor soil perturbations, and the enumeration procedure developed in this study should aid in following their responses to environmental stresses.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1975.tb05192.x
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  • 2
    Digitale Medien
    Digitale Medien
    Yield Constant for Acanthamoeba polyphaga in Batch and Continuous Culture (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . A two-stage chemostat modified to accommodate the growth of adhesive organisms was used to determine the yield constant, Y, of a representative soil amoeba, Acanthamoeba polyphaga, utilizing as its prey Pseudomonas paucimobilis. The first stage consisted of a glucose-limited bacterial culture in steady state. The second stage consisted of a simplified predator-prey system, nongrowing bacteria serving as the limiting substrate for amoebae. A refined methodology to more accurately determine Y was developed, and Y for Acanthamoeba polyphaga in batch and continuous culture was determined to be 19.1%.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb05323.x
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  • 3
    Digitale Medien
    Digitale Medien
    Trophic interactions in soils as they affect energy and nutrient dynamics. III. Biotic interactions of bacteria, amoebae, and nematodes (1977)
    Springer
    Microbial ecology 4 (1977), S. 361-371 
    Details
    ISSN: 1432-184X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Bacteria (Pseudomonas), amoebae (Acanthamoeba), and nematodes (Mesodiplogaster) were raised in soil microcosms with and without glucose additions. Nematode and amoebal grazing on bacteria significantly reduced bacterial populations by the end of a 24-day incubation period. Amoebal numbers decreased in the presence of nematodes with a corresponding increase in nematode numbers which reached a maximum of 230 nematodes/g of soil in the treatment with amoebae and glucose additions. After 24 days the nematode populations in the treatments without carbon additions were dominated by resistant dauer larvae indicating the unavailability of food. Although larval numbers were high in the treatments with glucose additions, the adult component of the population was still increasing at the end of the 24-day experiment. The effect of the presence of amoebae on nematode abundance was of the same magnitude as addition of 600Μg glucose-C.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02013279
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  • 4
    Digitale Medien
    Digitale Medien
    Filter-out-grazers (FOG): A filtration experiment for separating protozoan grazers in soil (1985)
    Springer
    Biology and fertility of soils 1 (1985), S. 73-79 
    Details
    ISSN: 1432-0789
    Schlagwort(e): Protozoa ; Ciliates ; flagellates ; Nitrogen and phosphorus mineralization ; Grazing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary In the present experiment, natural protozoan fauna and other microbial components in water extracts from shortgrass prairie soil were separated on the basis of size by differential filtration (8-, 5-, and 3-μm porosities). All extracts contained bacteria and fungi, along with a few very small flagellates (3-μm pore size filtrate); flagellates and a few small amoebae (5-μm pore size filtrate); and flagellates, small amoebae, and small ciliates (8-μm pore size filtrate). All microorganisms, except a few species of flagellates, were present in the centrifuge treatment. Each filtrate was added to sterile soil, and the population of each microbial group was determined after inoculation at intervals up to 80 days (at room temperature). Populations of all added groups decreased on initial addition to soil but then increased during the incubation. By following nitrogen, phosphorus, and CO2 dynamics, we observed impacts of protozoan grazing on bacteria, including mineralization of N from microbial biomass.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00255133
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