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  • 1
    Digitale Medien
    Digitale Medien
    20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway (1996)
    [s.l.] : Nature Publishing Group
    Nature 384 (1996), S. 276-279 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] We first analysed the growth characteristics of a cut4-533 fission yeast mutant in two culture media, YPD (Fig. la) and EMM2 (Fig. Id) at either 26 °C or 36 °C. The cells grew in EMM2 at both temperatures, and in YPD only at 26 °C. Growth was inhibited by ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/384276a0
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  • 2
    Digitale Medien
    Digitale Medien
    Zinc-finger motifs expressed in E. coli and folded in vitro direct specific binding to DNA (1988)
    [s.l.] : Nature Publishing Group
    Nature 332 (1988), S. 284-286 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Fig. 1 Expression in E. coli of the SWI5 polypeptide containing three zinc-finger motifs, a, Construction of the cleavable fusion-protein expression vector pLcIICLMSWIS. The fusion protein consists of the 31 amino-terminal residues of the AcII protein, 100 amino-acid residues encoded by the myosin ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/332284a0
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  • 3
    Digitale Medien
    Digitale Medien
    Cell biology Cytoskeleton in the cell cycle (2001)
    [s.l.] : Nature Publishing Group
    Nature 412 (2001), S. 291-292 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] The internal filamentous structures that support a cell and give it shape are more dynamic than their name — the cytoskeleton — might suggest. The cytoskeleton is constantly changing and rearranging, contributing to a variety of cellular functions. The two main components of the ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/35085684
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  • 4
    Digitale Medien
    Digitale Medien
    Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe (1984)
    Springer
    Current genetics 8 (1984), S. 93-97 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Integration vector ; Nucleolar DNA ; rRNA gene mapping ; Schizosaccharomyces pombe
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The major rRNA genes of the fission yeast Schizosaccharomyces pombe were mapped on chromosome III by plasmid integration. The integration vector YIp33 containing S. cerevisiae LEU2 gene was combined with the S. pombe rDNA. Since LEU2 complements S. pombe leu1 deficiency, it could be used as the genetic marker for integration. The 10.4 kb rDNA repeat contained ARS sequence, and therefore 2.4 kb and 0.7 kb subfragments not containing ARS were subcloned into YIp33 and transformed leu1 S. pombe cells to Leu+. Genetic analyses of the transformants indicated that the integrated rDNA resides in the long arm of the shortest chromosome III, tightly linked to ade5 (1.4 cM). This result is consistent with our previous finding that the DAPI-stained smallest chromosomes were associated with the nucleolus (Umesono et al. 1983).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00420224
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  • 5
    Digitale Medien
    Digitale Medien
    Meiosis-dependent mRNA splicing of the fission yeast Schizosaccharomyces pombe mes1 + gene (1994)
    Springer
    Current genetics 25 (1994), S. 497-503 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Fission yeast ; Intron ; Meiotic second division ; Splicing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The mes1 + gene of the fission yeast Schizosaccharomyces pombe is essential for the second meiotic division. We have cloned a 1.1-kb HindIII fragment containing mes1 + by complementation from an S. pombe genomic library. Sequencing of the genomic and cDNA fragments indicates the existence of one small intron of 75 nucleotides, although both the 5′ (G/GTTAGT) and 3′ (CAG/T) intron-exon junctions deviate from the consensus sequences proposed for S. pombe. The putative translation product of the mature mes1 + mRNA is a 11-kDa protein of 101 amino acids which has no significant homology to any previously-reported proteins. Disruption of mes1 has no effect on cell growth but causes an arrest of meiosis before the second meiotic division. Northern-blot analysis revealed that mes1 + was preferentially transcribed under conditions of nitrogen starvation. When a h 90 homothallic strain was shifted to a nitrogen-deficient medium, a pre-mRNA accumulated and then was gradually processed to generate a mature mRNA. This splicing did not occur in either a heterothallic haploid strain or in a homothallic mei2 mutant strain which was defective in the initiation of meiosis. Expression of the first exon alone was not able to suppress the mes1 null allele. These results indicate that mes1 + is required for the completion of meiosis, that splicing is required for the function of the mes1 + gene, and that this splicing requires the function of the mei2 + product.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00351668
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  • 6
    Digitale Medien
    Digitale Medien
    A telomerase mutant defective in sister chromatid separation at mitosis (1997)
    New York, NY : Wiley-Blackwell
    BioEssays 19 (1997), S. 557-559 
    Details
    ISSN: 0265-9247
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The telomere is a functional domain of the chromosome, located at the extreme ends, and is essential for normal chromosome stability. Chromosomes lacking telomeres are inherited improperly, and mutations in the telomeric repeat sequences are thought to lead to senescence and possibly to cancer. The molecular mechanisms maintaining chromosomes by telomeres, however, have been unclear. Results recently reported by Kirk et al.(1) offer an insight into new telomerase function. They have identified a novel telomerase mutation that blocks sister chromatid separation in mitosis.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bies.950190706
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