ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Annexin I, a member of the annexin family of Ca2+- and phospholipid-binding proteins, has been crystallized with the complete N-terminus. Annexins are structurally divided into a conserved protein core and an N-terminal domain that is variable in sequence and length. Three-dimensional structures of annexins comprising the protein core and a short N-terminal domain (annexins III, IV, V, VI, XII) or a truncated form almost completely lacking the N-terminal domain (annexins I and II) have been published so far. Here, the crystallization of annexin I comprising not only the core but also the complete N-terminal domain is reported. The crystals belong to the space group P212121, with unit-cell parameters a = 63.6, b = 96.3, c = 127.4 Å, and diffract to better than 2 Å. Assuming a molecular weight of 38.7 kDa for annexin I and an average value of 2.5 Å3 Da−1 for VM, two molecules per asymmetric unit are present.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900010301
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