ISSN:
1432-1424
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary An analytical technique is described for direct determination of the molecular composition of lipid bilayer membranes in aqueous solution. Membranes formed from chemically pure, radioactively labeled components, were sampled by pipetting a mercury droplet through the bilayer-water interface. During this procedure, the membrane remains intact but decreases in area with a concomitant increase in the area of the surrounding bulk phase. It is shown that each mercury droplet is covered with a fragment of the bilayer membrane in the form of a closed vesicle. The chemical composition of the bilayer is determined from an analysis of the readioactivity on the mercury droplet. Bilayers generated from glyceryl monooleate inn-decane orn-hexadecane contain (4.7±0.4)×1014 molecules of monoglyceride per cm2 and a minimum of (2.8±0.7)×1014 molecules of solvent (n-hexadecane) per cm2. It is estimated from these numbers that 37 vol % of the hydrocarbon core of the bilayer is occupied by solvent. The composition relationships between the bilayer and bulk membrane-forming solution were determined for mixtures of glyceryl monooleate (GMO) with cholesterol (Chol) or glyceryl monostearate (GMS). It was found that [GMS/GMO]bilayer≃[GMS/GMO]bulk, and [Chol/GMO]bilayer=0.5[Chol/GMO]bulk. While the molecular areas of glyceryl monooleate and glyceryl monostearate are unchanged in the mixed system, the average area for mixtures of cholesterol and glyceryl monooleate is decreased, suggesting a condensing effect of the sterol in the bilayer analogous to that observed in lipid monolayers.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01867845
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