ISSN:
1573-8221
Keywords:
interleukin-2 gene
;
protein factors
;
regulation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The nuclear protein factors which stimulate synthesis of mRNA of interleukin-2 (IL-2) in the system of surviving murine T lymphocytes are isolated from cells of the spleen and brain of immunized rats. The active fractions of spleen and brain (13–19 and 25 kD, respectively), which interact with the promoter-enhancer sequences of the IL-2 gene localized in the region from +39 to −141 base pairs, are isolated by the method of high performance liquid chromatography from the total nuclear extract. The induction of luciferase synthesis is shown for protein factor-treated Jurkat cells transformed with p-IL-2LUC recombinant plasmid.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02444307
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