ISSN:
1432-2013
Keywords:
Concentration-jump technique
;
Patch-clamp technique
;
Single smooth muscle cell
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract A new concentration-jump technique was devised for the rapid application of drugs to single, isolated cells attached to the base of the experimental chamber while recording from them with patch-clamp technique. Cells were placed in a micro-drop (less than 0.1 μl) in a small inner bath which was separated from an outer bath by a ring of “Sylgard” polymer. Stable whole-cell recordings were made in the micro-drop and rapid solution exchange took place when a much larger volume of test solution from the outer bath was flooded over the Sylgard ring and mixed with the micro-drop. Complete equilibration occurred within less than 10 ms.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00580285
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