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  • 1
    Electronic Resource
    Electronic Resource
    Expression of soybean b-1,3-endoglucanase cDNA and effect on disease tolerance in kiwifruit plants (1999)
    Springer
    Plant cell reports 18 (1999), S. 527-532 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAgrobacterium ; Kiwifruit ; β-1 ; 3-Endo-glucanase ; Transformation ; Disease tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Kiwifruit was transformed with a soybean β-1,3-endoglucanase (EC 3.2.1.39) cDNA under the control of the cauliflower mosaic virus (CaMV) 35S RNA promoter. The introduced gene was expressed in young leaves of the transformants. Assays of protein extracts from young leaves showed an increase in enzyme activity in many transformants, the transformant with the highest level of enzyme activity having an about sixfold increase over the control plants. When leaves from control and three transformants were inoculated with Botrytis cinerea, which causes gray mold disease, the disease lesion areas for two transformants were smaller than on control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050616
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Agrobacterium-mediated transformation and plant regeneration from hypocotyl segments of Japanese persimmon (Diospyros kaki Thunb) (1998)
    Springer
    Plant cell reports 17 (1998), S. 435-440 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAgrobacterium ; Hypocotyl ; Japanese persimmon ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hypocotyl segments from the seeds of Japanese persimmon (Diospyros kaki Thunb) were cultured on a modified Murashige and Skoog medium supplemented with N-(2-chloro-4-pyridyl)-N′-phenylurea, zeatin or 6-benzylaminopurine. The highest frequency of shoot regeneration was observed when the segments were cultured on medium containing 2 mg/l of zeatin. This culture system was adapted to Agrobacterium-mediated transformation. The hypocotyl segments were inoculated with Agrobacterium tumefaciens strains harboring binary vectors, which contained the neomycin phosphotransferase II gene and the β-glucuronidase gene. Regenerated shoots were selected on a medium containing kanamycin. Histochemical GUS assay showed that the shoots regenerated from the segments inoculated with EHA101/pSMAK251 expressed the gus gene. The presence and integration of the gus gene was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. The regeneration frequency of transformed shoot was 11.1%. The transgenic shoots were rooted and developed into whole plants within 4–5 months.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050421
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    Paper (German National Licenses)
    Fulltext
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