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Digitale Medien

The promoter of the geneItr1 from barley confers a different tissue specificity in transgenic tobacco (1995)

Diaz, I. ; Royo, J. ; O'Connor, A. ; [weitere]

Springer

Molecular genetics and genomics 248 (1995), S. 592-598

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ISSN: 1617-4623

Schlagwort(e): Barley trypsin inhibitor ; Promoter ; Tissue-specific expression ; Transgenic tobacco

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Tissue-specific expression of the gene coding for trypsin inhibitor BTI-CMe in barley (Itr1) occurs during the first half of endosperm development. In transgenic tobacco, theItr1 promoter drives expression of the β-glucuronidase reporter gene not only in developing endosperm but also in embryo, cotyledons and the meristematic intercotyledonary zone of germinating seedlings. A promoter fragment extending 343 bp upstream of the translation initiation ATG codon was sufficient for full transgene expression, whereas, the proximal 83 bp segment of the promoter was inactive. Possible reasons for the differences in expression patterns are discussed.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02423455

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