ISSN:
1432-0428
Keywords:
Type 1 diabetes
;
islet cell surface antibodies
;
cytotoxic antibodies
;
antibody-dependent cell-mediated cytotoxcity
;
human pancreatic B cells
;
islet cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Sera containing islet cell surface antibodies show a complement-dependent cytotoxic reaction against islet cells, but it has not yet been clarified whether islet cell surface antibodies exhibit cell-mediated cytotoxicity to these cells. By 51Cr release assay we investigated whether islet cell surface antibodies showed a cytotoxic reaction to human pancreatic B cells (JHPI-1 clone) in the presence of normal human lymphocytes. The sera from 14 islet cell surface antibody-positive, 16 islet cell surface antibody-negative Type 1 (insulin-dependent) diabetic patients and 18 islet cell surface antibody-negative healthy subjects were studied. Four sera containing islet cell surface antibodies showed specific cytotoxicity above the mean +3SD value of healthy subjects, and the mean specific cytotoxicity of islet cell surface antibody-positive sera differed significantly from that of both islet cell surface antibody-negative groups. These results suggest that this cell-mediated cytotoxic mechanism may play an important role in the pathogenesis of Type 1 diabetes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00252259
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