ISSN:
1573-5060
Schlagwort(e):
Poa pratensis
;
Kentucky bluegrass
;
protoplast
;
plant regeneration
;
suspension culture
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
Notizen:
Summary A procedure for the regeneration of callus and albino plantlets from protoplasts of Kentucky bluegrass (Poa pratensis L.), cultivar Kimono, is described. Protoplasts were isolated from a suspension culture with shoot-forming ability, which had been initiated from immature inflorescence derived callus. Protoplasts were cultured in modified Kao & Michayluk (KM8P) liquid medium. Plantlets were recovered from protoplast-derived callus growing on hormone-free modified Murashige & Skoog (MS) medium. While the plantlets derived from the original callus cultures were green, those from suspension cultures and suspension culture derived protoplasts were albino.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00043380
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