ISSN:
1434-4475
Keywords:
Gastrointestinal hormone
;
Human-secretin
;
Synthetic peptide factors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The synthesis of the heptacosapeptide amide with the primary structure of Human-secretin is described. For this purpose 7 fragments were designed, i.e. H-Gly-Leu-Val-NH2 〈25–27b〉,Z-Arg(Z 2)-Leu-Leu-Gln-OH 〈21–24〉,Z-Arg(Z 2)-Leu-Gln-OH 〈18–20〉,Z-Arg(Z 2)-Glu(OtBu)-Gly-Ala-OH 〈14–17〉,Z-Arg(Z 2)-Leu-OH 〈12–13〉,Z-Thr(tBu)-Ser(tBu)-Glu(OtBu)-Leu-Ser(tBu)-OH 〈7–11〈,Adoc-His(Adoc)-Ser(tBu)-Asp(OtBu)-Gly-Thr(tBu)-Phe-OH 〈1–6〈 these fragments were consequently assembled to the overall protected total sequence using the Wünsch/Weygand-method with dicyclohexylcarbodiimide. After deprotection by exposure to trifluoroacetic acid in presence of 1,2-ethanedithiol and water as scavenger, the isolated crude product was purified by column chromatography on CM-Sepharose, fast flow. This synthetized Human-secretin showed the full biological activity in comparison to Porcine-secretin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00819525
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