ISSN:
1432-072X
Keywords:
Azotobacter
;
Nitrogenase
;
Iron Sulfur Proteins
;
Properties
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The purified Mo-Fe protein and Fe protein of nitrogenase from Azotobacter vinelandii have molecular weights (MW) of about 216000 and 64000, respectively. The Mo-Fe protein is composed of subunits of about 56000 MW, and the Fe protein has 2 equivalent subunits of about 33000 MW. The isoelectric point of the Mo-Fe protein is 5.2 and that of the Fe protein is 4.7. Amino acid compositions reflect the acidic nature of the proteins. The Mo-Fe protein yielded 24 atoms of Fe, 20 atoms of acid-labile sulfide and 1.54 atoms of Mo per molecule. Analysis of the Fe protein showed 3.45 atoms of Fe per molecule.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425272
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