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Digitale Medien

Presence of cells and fibers immunoreactive toward antibodies to different peptides or amine in the digestive tract of the snailHelix aspersa (1991)

Marchand, C. R. ; Colard, C.

New York, NY : Wiley-Blackwell

Journal of Morphology 207 (1991), S. 185-190

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ISSN: 0362-2525

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: An immunocytological study of four different parts of the gut of Helix aspersa clearly demonstrates the presence of many cells and fibers immunoreactive toward antibodies directed to vertebrate (α, β-endorphin, α, β-MSH, ACTH 1-24 and ACTH 17-39, met-enkephalin, somatostatin, insulin, glucagon, P.P., serotonin) or invertebrate (FMRF-amide) peptides.These results are evidence of the presence of different substances related to known peptides or amines in the epithelial and connective tissue cells and nerve fibers of the snail gut. Immunocytochemistry may help to elucidate the morpho-functional characteristics of the enteroendocrine cells of H. aspersa.

Zusätzliches Material: 16 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jmor.1052070209

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Digitale Medien

Immunofluorescent localization and ultrastructural characterization of gonadotrophe cells in the adenohypophysis of the barbary drake (Cairina moschata L.) using anti-chicken LH serum (1977)

Marchand, C. R. ; Sharp, P. J.

Springer

Cell & tissue research 181 (1977), S. 531-544

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ISSN: 1432-0878

Schlagwort(e): Adenohypophysis, duck ; Gonadotrophes ; Immunofluorescence ; Ultrastructure

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary An indirect immunofluorescence technique and an anti-chicken LH serum were used to localize cells in the adenohypophyses of drakes at different stages of their breeding cycle, after castration, and after castration combined with thyroxine treatment. Immunofluorescent cells were distributed throughout both lobes of the adenohypophyses from control and experimental birds and were shown to be alcian blue positive, PAS negative, basophiles. Immunofluorescent cells were as numerous in castrated birds as in castrated birds treated with thyroxine. Adjacent thin and semi-thin sections were used to study the cells binding anti-LH serum at light microscope and ultrastructural levels. The cells contained spherical granules with variable densities and diameters ranging between 40 and 280 nm in the rostral (= cephalic) lobe, and between 60 and 260 nm in the caudal lobe. The light microscope and ultrastructural observations showed that the anti-LH serum binds to cells which have been classified by other authors in the Pekin duck, quail and pigeon as TSH producing delta cells. The experimental technique used did not permit a distinction to be made between cells producing FSH and LH.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00221774

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