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Digitale Medien

Male-sterile chicory cybrids obtained by intergeneric protoplast fusion (1993)

Rambaud, C. ; Dubois, J. ; Vasseur, J.

Springer

Theoretical and applied genetics 87 (1993), S. 347-352

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ISSN: 1432-2242

Schlagwort(e): Cichorium intybus ; Protoplast fusion ; Cytoplasmic male sterility ; Mitochondrial DNA

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Male-sterile chicory plants were obtained by fusion of chicory mesophyll protoplasts and hypocotyl protoplasts derived from male-sterile sunflower plants. The protoplasts of both species were fused by the PEG method and the products were selected manually and cultivated at very low density in a liquid medium. Three to twenty percent of the heterokaryocytes divided and evolved into microcalli, then into calli where budding could be induced. The mitochondrial genome of ten male-sterile or totally sterile plants was studied. Restriction endonuclease profiles of mitochondrial DNA and molecular hybridization with specific genes of the mitochondrial genome used as probes indicated that mitochondrial DNA rearrangement had occurred between sunflower and chicory and the intensity of the rearrangements correlated with the degree of sterility of the different plants.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01184922

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Digitale Medien

The induction of tetraploidy in chicory (Cichorium intybus L. var. Magdebourg) by protoplast fusion (1992)

Rambaud, C. ; Dubois, J. ; Vasseur, J.

Springer

Euphytica 62 (1992), S. 63-67

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ISSN: 1573-5060

Schlagwort(e): chicory ; Cichorium intybus ; protoplast fusion ; tetraploids

Quelle: Springer Online Journal Archives 1860-2000

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft

Notizen: Summary Fusions of chicory leaf protoplasts were realized in order to obtain a higher number of tetraploid plants compared to the traditional doubling techniques with colchicine. Thanks to the fusion technique, using a mixture of PEG, DMSO and a solution of CaCl2 (pH 10.5), 25% tetraploid (2n=36) plants were obtained. Among the 167 regenerated plants, only one aneuploid of a near tetraploid level (2n=33) was identified.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00036089

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