ISSN:
1040-452X
Keywords:
IGF-IA
;
Long 3′-end
;
Expression
;
Transcripts sizes
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
A cDNA clone of 525 bp corresponding to the 3′-untranslated region of insulin-like growth factor-I was isolated from a human placenta library. The sequence of this clone extended 200 nucleotides downstream from the previously reported 3′-end of IGF-IA cDNA, indicating the existence of IGF-IA transcripts having an even larger 3′-untranslated region. By using this clone for RNA transfer blot hybridization, it was shown that this longer 3′-untranslated region is included in the 7.5- and 5.0-kb transcripts, but not in the 1.1- and 0.9-kb transcripts. It is also apparent that transcripts bearing the extended 3′-untranslated sequence are highly expressed in human placenta.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1080290305
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