ISSN:
1432-0711
Keywords:
Key words: Photodynamic therapy
;
Photosensitizers
;
m-THPC
;
Breast cancer
;
In vitro
;
Adenosine triphosphate cell viability assay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract. Background: Photodynamic therapy (PDT) might be of clinical value for patients with breast cancer with local recurrences or metastasis. However, there is a need for improved photosensitizers that are effective in combination with laser light and have few, if any, side-effects. We evaluated in vitro the effectiveness of a second generation photosensitizer by testing the influence of laser light on cell cultures of a human breast carcinoma cell line, incubated with meta-tetrahydroxyphenylchlorin (m-THPC) (=Temoporfin®). Experimental design: Five thousand MCF-7 cells were plated in 96-well plates. Forty-eight hours before laser treatment, the cells were plated to achieve a monolayer configuration. Twenty-four hours after plating, they were incubated with m-THPC. On day 6 after treatment with m-THPC we lysed the cells to extract the intracellular ATP that correlates with the number of living cells. The ATP-CVA was used to assess the cytotoxicity of the tested photosensitizer m-THPC at various concentrations and the relevant laser light alone prior to their combination after six days of culture. Results: We found a dose-response for m-THPC alone ranging from 2 to 16 µg/ml. The calculated inhibition concentration to produce 50% cell kill (IC50) was 4.55 µg/ml. We also observed a very low cytotoxicity for laser irradiation alone but a very strong cell kill for the combination of m-THPC together with laser light. Conclusions: PDT gave almost total cell kill at m-THPC concentrations that are not toxic in vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00634488
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