Electronic Resource
[S.l.]
:
American Institute of Physics (AIP)
Review of Scientific Instruments
68 (1997), S. 1886-1888
ISSN:
1089-7623
Source:
AIP Digital Archive
Topics:
Physics
,
Electrical Engineering, Measurement and Control Technology
Notes:
An improvement to the Lewis–Kliger method for measuring transient circular dichroism on the nanosecond time scale is described. The method uses a single-probe beam that is split into two different beams of plane polarized light entering the sample and a retarder from opposite directions in different succession. Rochon polarizers are used as high-quality polarizing beam splitters to select the slow axis component of the emerging elliptical polarized light beams. The intensities of the light beams are determined by an imaging spectrograph coupled to an intensified charge coupled device detector. The split beam method reduces the need for very precise calibration of the central strain plate acting as a retarder and controlling the ellipticity of the probe light. The necessary calculations are simple and can be shown to be equivalent to the formulas derived by Lewis and Kliger. The static CD spectrum of vitamin B12 is presented and compared to a spectrum obtained with a commercial instrument and standard technique. The time resolution of the instrument is demonstrated by observation of photobleaching of carbon monoxy myoglobin from horse heart muscle. © 1997 American Institute of Physics.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1063/1.1147962
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