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  • 1
    Electronic Resource
    Electronic Resource
    Bradford : Emerald
    Engineering computations 13 (1996), S. 4-30 
    ISSN: 0264-4401
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Technology
    Notes: Outlines a general methodology for the solution of the system of algebraic equations arising from the discretization of the field equations governing coupled problems. Considers that this discrete problem is obtained from the finite element discretization in space and the finite difference discretization in time. Aims to preserve software modularity, to be able to use existing single field codes to solve more complex problems, and to exploit computer resources optimally, emulating parallel processing. To this end, deals with two well-known coupled problems of computational mechanics - the fluid-structure interaction problem and thermally-driven flows of incompressible fluids. Demonstrates the possibility of coupling the block-iterative loop with the nonlinearity of the problems through numerical experiments which suggest that even a mild nonlinearity drives the convergence rate of the complete iterative scheme, at least for the two problems considered here. Discusses the implementation of this alternative to the direct coupled solution, stating advantages and disadvantages. Explains also the need for online synchronized communication between the different codes used as is the description of the master code which will control the overall algorithm.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Sensitization to soybean hull (SH) allergens occurs in subjects from Argentina, a soybean producer country. However, the causative allergens have not been identified. The purposes of this study are to: (i) identify the SH allergens using sera of 29 subjects with asthma and/or allergic rhinitis from Argentina exposed to soybean dust who have a positive (weal with SH/weal with histamine ≥ 0.5) skin prick test to SH; and (ii) determine the N-terminal amino acid sequence of a major 50 K SH allergen that sensitizes this population.Methods All sera were assayed for specific IgE (RIA), IgG4 (ELISA), and IgE and IgG4-Western blots. A sera pool from 10 healthy subjects was a negative control. N-terminal amino acid sequencing was performed by the Edman degradation method.Results Positive specific IgE only was found in 12/29 (41.4%), IgG4 in 3/29 (10.3%), and both IgE and IgG4 in 14/29 (48.3%) sera. IgE-Western blot demonstrates: (i) an allergen, MW 50 K (51.7% binding); (ii) one or two distinct allergens, MW 〈 20.2 K (72.4% binding), depending on the sera; and (iii) 1–5 additional IgE binding proteins, MW 〉 20.2 to 〈 46.9 K (41.4% binding), depending on the sera. IgG4-Western blot demonstrates: (i) a band, MW 70K (31% binding); (ii) a band, MW 50 K (17.2% binding); (iii) one or two additional bands, MW 〈 20.2 K (51.7% binding), depending on the sera; and (iv) a band, MW 〉 20.2 to 〈 28.5 K (20.7% binding). The 50 K allergen N-terminal amino acid sequence of the first 17 amino acids indicates a significant homology with chlorophyll A-B binding protein precursors from tomato, spinach, and petunia.Conclusions Specific IgE and IgG4 to SH are common in sera from allergic individuals living in rural areas in Argentina. SH contain an IgE binding protein, MW about 50 K, not previously described. Sensitization to this allergen is common in subjects who are repeatedly exposed to soybean dust inhalation.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background A low MW allergen from soybean hull, Gly m 1, with two isoallergens, Gly m 1 A and Gly m 1 B, was associated with the asthma outbreaks that occurred in Cartagena, Spain. Using sera of asthmatic epidemic patients (AEP) from Barcelona, three main soybean hull allergens, two of them with MWs atid pIs identical to those reported for Gly m 1 A and Gly m 1 B, were identified.Objective The purpose of this study was to purify and to study the N-terminal amino acid sequence of the third allergen, which has a MW of 8 kDa.Method The purification procedure combined the double dialysis method and preparative isoelectofocusing (IEF). Specific IgE determination to the fractions obtained demonstrated three peaks, one of them corresponding to the 8 kDa allergen. The pooled fractions containing this allergen were studied by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), SDS-PAGEAVestern blot and IEF/Westem blot. Only a band with a MW of 8 kDa and a pI of 6 was obtained. Its allergenic activity was measured and it was demonstrated that the allergenicity of soybean hull correlates with the presence of the 8 kDa allergen. The N-terminal amino acid sequence of the first 20 amino acids, which was registered at the PIR Data Submission as the N-terminal partial sequence of Gly m 2, was determined according the Edman degradation method.Results Gly m 2 N-terminal amino acid sequence lacks homology with that reported for the allergen Gly m 1 but has a homology of 71% with a storage protein from cotyledon of Vigna radiata (cow pea) and 64% with a‘disease response protein’ from Pisum sativum (green pea). These results suggest that Gly m 2 in soybeans could protect against diseases which affect soybean plants.Conclusion This study demonstrates the existence of another soybean hull allergen, Gly m 2, partially responsible for the soybean asthma outbreaks that occurred in Barcelona, Spain.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: The effectiveness of acaricides in homes is controversial.Objective: To determine whether disodium octaborate tetrahydrate (DOT) combined with vacuuming lowers dust mite numbers and their allergens in carpets and sofas.Methods: A 6-month study was carried out with 93 homes, which were randomized into three groups: (i) active, received DOT; (ii) placebo, received water; and (iii) control, received no application. Active and placebo homes were vacuumed weekly. Dust was collected from carpets and sofas at the start of the study and every 2 months thereafter and quantified for live, total mites, and mite allergen levels.Results: At 2 months, live mite numbers in active carpets were 3 ± 1, in placebo carpets 129 ± 48, and in control carpets 177 ± 39 mites/g. The corresponding numbers in sofas were 3 ± 2, 81 ± 31, and 134 ± 45 mites/g, respectively (P 〈 0.001 active vs placebo and vs. control). Live mites in carpets and sofas remained lower in the active group at 6 months (P 〈 0.001). Total mites in active carpets decreased from 555 ± 69 at baseline to 223 ± 32 mites/g at 6 months (P 〈 0.001) and mite allergen levels from 1.36 ± 0.13 to 0.85 ± 0.16 μg/g (P 〈 0.001). Total mites in active sofas remained unchanged, but mite allergen levels decreased from 1.48 ± 0.25 at baseline to 0.7 ± 0.15 μg/g at month 6 (P 〈 0.05).Conclusion: DOT kills mites in carpets and sofas, and, combined with vacuuming, effectively reduces total mites in carpets and mite allergen levels in carpets and sofas.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Munksgaard International Publishers
    Allergy 58 (2003), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Soybean hulls (SHs) cause respiratory allergies. This study investigates the allergenicity of soybean varieties (SVs) by in vivo and in vitro tests.Methods:  Ten SVs were studied: (a) five with a proved clinical relevance (SVs 1, 2, 3, 4, 5), the last four with a ‘dull’ phenotype; (b) five of undetermined relevance, three of them (SVs 6, 7, 8) with a ‘shiny’ phenotype, and two (SVs 9 and 10) with a ‘dull’ phenotype. Extracts from all 10 SVs were used to skin prick test (SPT) 21 subjects sensitized to SHs. Positive and negative sera pools prepared from sera of subjects sensitized or not to SHs, respectively, were utilized to perform in vitro experiments (specific IgE and IgG4 determinations, SDS-PAGE/IgE-Western blot, and IgE-inhibition).Results:  In this study, it was found that 52.4, 52.4, 57.1, 71.4, 80.9, 42.9, 57.1, 71.4, 52.4, and 38.1% subjects had a positive SPT with SVs 1–10, respectively (P NS). Specific IgE values to SVs 1–10 obtained with the positive pool are 28.3, 26.4, 29.9, 28.3, 26.8, 4.8, 13.4, 6.7, 24.7, and 17.5% total counts bound, respectively; and specific IgG4 values 0.851, 0.818, 0.721, 1.609, 0.789, 0.617, 0.662, 0.0, 1.127, and 0.934 OD units, respectively; the microgram of protein required to produce 50% inhibition are 2.5, 3.7, 4.5, 2.4, 5, 39.8, 25.2, 25.1, 4.5, and 8.9, respectively. A 7-kDa band is present in all SVs except in those with a ‘shiny’ phenotype.Conclusions:  The SVs with a ‘shiny’ phenotype contain less allergens than the other SVs studied, as determined by in vitro tests. However, SPT results with the SVs do not differ. Genetic screens should be devised to select plants with reduced, preferably absent, allergenicity, but with a high nutritional value, and this allergenicity should be studied utilizing in vivo and in vitro tests.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science, Ltd
    Allergy 57 (2002), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 23 (1996), S. 787-809 
    ISSN: 0271-2091
    Keywords: operator-splitting ; general algorithm ; characteristic-Galerkin ; laminar/turbulent ; incompressible ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In an earlier paper, Zienkiewicz and Codina (Int. j. numer. methods fluids, 20, 869-885 (1995)) presented a general algorithm for the solution of both compressible and incompressible Navier-Stokes equations. The algorithm, based on operator splitting, permits arbitrary interpolation functions to be used while avoiding the Babŭska-Brezzi restriction. In addition, its characteristic based approach introduces a form of rational dissipation. Zienkiewicz et al. (Int. j. numer. methods fluids, 20, 887-913 (1995)) presented the application of this algorithm in its fully explicit form to various inviscid compressible flow problems. They also presented two incompressible flow problems solved by the fully explicit form, employing a pseudo compressibility. The present work deals with the application of the above algorithm it its semi-implicit form to some incompressible flow benchmark problems. Further, it extends the methodology to turbulent flows by employing both one, and two equation turbulence models. A comparison of results with earlier investigations is presented. Other issues addressed in this study include the effect of additional diffusion terms present in the scheme for both laminar and turbulent flow problems and some practical difficulties associated with local time stepping.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 20 (1995), S. 869-885 
    ISSN: 0271-2091
    Keywords: compressible flow ; CFD (computational fluid dynamics) ; finite elements in fluids ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The paper outlines the formulation of a novel algorithm which can be used for the solution of both compressible and incompressible Navier-Stokes or Euler equations. Full incompressibility can be dealt with if the algorithm is used in its semi-explicit form and its structure permits arbitrary interpolation functions to be used avoiding the Babuška-Brezzi restriction. In a fully explicit version it introduces a rational form of balancing dissipation avoiding the use of arbitrary parameters and forms for this.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 20 (1995), S. 887-913 
    ISSN: 0271-2091
    Keywords: compressible flow ; CFD (computational fluid dynamics) ; finite elements in fluids ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The algorithm introduced in Part I of this paper is applied in its explicit form to a variety of problems in order to demonstrate its wide range of applicability and excellent performance. Examples range from nearly incompressible, viscous, flows through transonic applications to high speed flows with shocks. In most examples linear triangular elements are used in the finite element approximation, but some use of quadratic approximation, again in triangles, indicates satisfactory performance even in the case of severe shocks.
    Additional Material: 23 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 28 (1998), S. 1325-1353 
    ISSN: 0271-2091
    Keywords: finite elements ; compressible flow ; artificial viscosity ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The performance of different shock capturing viscosities has been examined using our general fluid mechanics algorithm. Four different schemes have been tested, both for viscous and inviscid compressible flow problems. Results show that the methods based on the second gradient of pressure give better performance in all situations. For instance, the method constructed from the nodal pressure values and consistent and lumped mass matrices is an excellent choice for inviscid problems. The method based on L2 projection is better than any other method in viscous flow computations. The residual based anisotropic method gives excellent performance in the supersonic range and gives better results in the hypersonic regime if a small amount of residual smoothing is used. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 23 Ill.
    Type of Medium: Electronic Resource
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