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  • 1
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Solid state phenomena Vol. 78-79 (Apr. 2001), p. 341-344 
    ISSN: 1662-9779
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0630
    Keywords: 61.70 ; 61.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Transmission electron microscopy, optical reflection and channeling effect measurements are employed to investigate disorders in 30 keV, high dose (3×1016ions/cm2) and high current (≦5 mA) phosphorus as-implanted silicon with (111), (100), and (110) orientation as a function of temperature rise (100–850°C) by the beam heating effect during implantation. Temperature rise below 400°C results in continuous amorrphous layer formation. This contrasts with results of the recovery into single crystals for temperature rise samples above 500°C, regardless of wafer orientation. Secondary defects (black-dotted defects, dislocation loops and rodlike defects) are formed in singlecrystal recovery samples, having a deeper distribution in (110) wafers and a shallower distribution in (111) and (100) wafers. Rodlike defects observed in 850°C samples are of “vacancy” type and have the largest density in (110) wafers.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 112 (1972), S. 113-123 
    ISSN: 1432-1076
    Keywords: Urinary Enzymes in Newborns ; In Childhood Nephritis ; Nephrosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir haben im Urin von insgesamt 350 gesunden und kranken Kindern die Aktivität von 7 Enzymen und deren Isoenzymeigenschaften untersucht. Bei Neugeborenen ist die höchste Aktivität der alkalischen Phosphatase, der Glutamin-Oxalacetat-Transaminase und des Lysozyms am 1. und 2. Tag nach der Geburt nachzuweisen. Bei mehr als der Hälfte der Kinder mit akuter Glomerulonephritis sind die Aktivitäten der alkalischen Phosphatase, der Glutamin-Oxalacetat-Transaminase und der Lactat-Dehydrogenase pathologisch erhöht und gehen im Heilungsstadium allmählich auf normale Werte zurück. Auch bei orthostatischer Proteinurie sind die Aktivitäten für diese Fermente sowie für β-Glucuronidase erhöht. Demgegenüber sind bei Kindern mit nephrotischem Syndrom die Fermentaktivitäten meistens tiefer. Pathologisch erhöhte Enzymaktivitäten bestehen ferner bei Kindern mit Leukämie, Neuroblastom, Pneumonie, Hyperthyreoidismus, Hyperbilirubinämie, Asthma bronchiale und Diabetes mellitis. Zwischen den Aktivitäten eines bestimmten Ferment im Urin und im Serum besteht keine Korrelation.
    Notes: Abstract The determination of serum enzyme levels has been increasingly used in diagnosis of children's diseases. However, urinary enzymes have been little studied and many problems remain to be elucidated. As a possible guide to differential diagnosis and prognosis of children's diseases, the urinary enzyme levels were determined in 350 children with and without diseases. The urinary levels of lysozyme (MG=14,000), amylase (MG=45,000), alkaline phosphatase (AP, MG=100,000), glutamate transaminase (GOT, MG=100,000–120,000), lactic dehydrogenase (LDH, MG=140,000), leucine aminopeptidase (LAP, MG=300,000) and β-glucuronidase (MG=300,000) were measured simultaneously in each child. In the neonatal period the highest activities of alkaline phosphatase, glutamate transaminase and lysozyme were observed on the first and second day after birth. The urinary levels of alkaline phosphatase, glutamate transaminase and lactic dehydrogenase were elevated in more than half of the cases in the acute phase of glomerulonephritis, and gradually decreased down to normal levels during recovery. High levels of alkaline phosphatase, glutamate transaminase, lactic dehydrogenase and β-glucoronidase were found in some cases of orthostatic proteinuria. In nephrosis, however, the enzyme activities were mainly low. Abnormally high enzyme levels were observed in leukemia, neuroblastoma, pneumonia, hyperthyreoidism, hyperbilirubinemia, bronchial asthma, and diabetes mellitus. No correlation was found between the enzyme activities in the urine and serum.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 137 (1981), S. 17-21 
    ISSN: 1432-1076
    Keywords: Menkes kinky hair syndrome ; Copper and zinc contents ; Copper and zinc proteins ; Metalloprotein ; Metallothionein-like protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Copper and zinc concentrations, and the nature of the copper- and zinc-binding proteins, were studied using tissues from a Menkes patient who had been given intravenous infusions of cupric acetate. The liver and brain copper contents were lower than in an untreated, non-Menkes control, and the spleen, intestine and kidney showed higher copper concentrations than control tissues. Zinc concentrations in all the organs (except the kidneys) from the Menkes patient were slightly lower than those the control child. Using Sephadex G-75 column chromatography of cytosols (105,000 x g supernatant), three copper- and zinc-containing peaks were eluted. In all the Menkes tissues studied, copper was prominent in peak 3. On the other hand, peak 3 was the smallest and peak 1 was the largest in the control tissues. Zinc predominated in peak 1 in both Menkes and control tissues, except for Menkes kidney. In this tissue peak 3 was again the largest. The copper- and zinc-binding material in peak 3 fractions from Menkes kidney was chromatographed on DEAE-cellulose columns. Three copper- and zinc-containing peaks were observed at the same positions as metallothionein from human adult kidneys.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1076
    Keywords: Homocystinuria ; Homocystine ; Methionine ; Cystathionine synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A boy with homocystinuria due to cystathionine synthase deficiency was found to have hypermethioninaemia by neonatal blood screening, but was not diagnosed as homocystinuric until 3 months of age because urinary homocystine was not detected by the cyanidenitroprusside test or on two examinations with a sensitive amino acid autoanalyser. These findings indicate that tests for urinary homocystine should be made repeatedly with an amino acid auto-analyser in newborn infants with hypermethioninaemia until the enzyme defect is identified.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 112 (1972), S. 124-132 
    ISSN: 1432-1076
    Keywords: Urinary Enzymes and Isozymes ; Experimental Rat Nephropathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wurden Urin-, Serum-und Nierenenzyme von Wistarratten nach experimenteller Nierenschädigung untersucht und die Aktivitäten von 6 Urinenzymen (Lysozym, alkalische Phosphatase, Glutamin-Oxalacetat-Transaminase, Lactat-Dehydrogenase, Leucin-Aminopeptidase und β-Glucuronidase) bestimmt und gleichzeitig auch Isoenzyme (alkalische Phosphatase, Glutamin-Oxalacetat-Transaminase und β-Glucuronidase) untersucht. Wir finden im Aktivitätsverlauf der Urinenzyme und der Isoenzyme deutliche Unterschiede zwischen den Ratten, denen Nephrotoxin und denen Aminonucleosid injiziert wurde. Bei den Isoenzymen der verschiedenen Urinenzyme war bei beiden Gruppen ein deutlicher Unterschied zu beobachten. Das Muster der Isoenzyme ist bei Urin-und Nierenfermenten ähnlich, weicht aber von dem der Serumfermente ab.
    Notes: Abstract The origin of urinary enzymes is obscure. We therefore determined the levels of urinary, serum and kidney enzymes (lysozyme, amylase, alkaline phosphatase, glutamate transaminase, lactic dehydrogenase, leucine aminopeptidase and β-glucuronidase) and isozymes (alkaline phosphatase, glutamate transaminase, leucine aminopeptidase and β-glucuronidase) in Wistar rats with nephrotoxin nephritis or aminonucleoside nephrosis. The urinary enzyme activities varied in several ways in rats with nephrotoxin nephritis and with aminonucleoside nephrosis. A significant difference in the isozymes was found in the two groups. The isozyme-pattern of urinary enzymes resembles that of kidney enzymes, but not that of serum enzymes. These findings indicate that the urinary enzymes are mainly formed by destruction and renewal of renal tissue.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 134 (1980), S. 205-210 
    ISSN: 1432-1076
    Keywords: Menkes kinky hair syndrome ; Menkes fibroblasts ; Copper metabolism ; Metalloprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Menkes fibroblast cultures were established and copper metabolism was investigated. Menkes fibroblasts contained four to six times higher concentrations of copper than control cells. In Menkes cells more than 90% of the intracellular copper was present in cytosol (105,000 x g supernatant fraction); in control fibroblasts the corresponding value was about 67%. During cultivation of fibroblasts in medium supplemented with 100 ng/ml of copper (270 ng CuCl2·2H2O /ml), the amount of copper increased continuously in Menkes cells, at least up to 4 days, while in control cells in reached a maximum after 24 h, followed by a gradual decrease. When the medium was replaced with one without copper chloride, copper concentrations in Menkes cells returned to the original level in three days, whereas those in control cells returned to the normal level in one day. Using Sephadex G-75 column chromatography of cytosol, two copper-containing peaks were observed (peaks 1 and 3, corresponding to the peaks from rat liver cytosol). Approximately 75% of the copper in the cytosol from Menkes cells was eluted in peak 3. The corresponding copper peak was very small in control cells. Copper peaks 1 and 3 from both cells increased after treating cells with copper chloride and the increase was inhibited by cycloheximide, an inhibitor of protein biosynthesis. Metallothionein purified from human kidneys was eluted as a single copper-protein from a Sephadex G-75 column in the same fractions as peak 3. SDS polyacrylamide-slab gel electrophoresis of the purified metallo-thionein and the material eluted in peak 3 from Menkes fibroblasts showed single peaks for copper at identical migration distances.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Solid state phenomena Vol. 78-79 (Apr. 2001), p. 345-348 
    ISSN: 1662-9779
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 561-565 (Oct. 2007), p. 1181-1184 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Trends in Biochemical Sciences 10 (1985), S. 122-124 
    ISSN: 0968-0004
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
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