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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 42 (1986), S. 150-151 
    ISSN: 1420-9071
    Keywords: Arylsulfatase A ; arylsulfatase B ; 4-methylumbelliferyl sulfate ; rodent ; bovine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rodent and bovine arylsulfatase B hydrolyze 4-methylumbelliferyl sulfate (4MUS) 10- to 30-fold more efficiently than arylsulfatase A. Therefore, 4MUS grossly underestimates arylsulfatase A activity in the presence of excess arylsulfatase B.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Keywords: Keywords Leptin ; leptin receptor ; Ob-R ; obesity ; sequence variant.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Leptin is an adipocyte-derived blood-borne satiety factor that acts on its cognate leptin receptor (Ob-R) in the hypothalamus, thereby regulating food intake and energy expenditure. To explore whether mutations in the Ob-R gene cause obesity in humans, we have searched for mutations in the gene for Ob-Rb, a biologically active receptor isoform, in obese Japanese subjects. We have also examined associations between such mutants and obesity in the Japanese. Genomic DNAs were used as templates in polymerase chain reaction (PCR) with primers selected to amplify exons 2 to 20 of the human Ob-Rb gene. Direct sequence analysis of the PCR products revealed 7 nucleotide sequence variants (Lys109Arg, Gln223Arg, Ser343Ser, Ser492Thr, Lys656Asn, Ala976Asp, and Pro1019Pro) in the Ob-Rb coding region from 17 obese Japanese subjects with a family history of obesity (BMI 39.3 ± 8.4 kg/m2). No missense and nonsense mutations were found such as those in Zucker fatty (fa/fa) rats and Koletsky (fa k /fa k) rats. Nucleotide substitutions occurred at relatively high frequencies at codons 109, 223, 976, and 1019 (79, 91, 100, and 85 %, respectively). Allele frequency of each variant determined by PCR-RFLP and PCR-single strand conformation polymorphism analyses showed no significant differences between 47 obese (BMI 35.1 ± 6.5 kg/m2) and 68 non-obese (BMI 21.6 ± 2.2 kg/m2) subjects. The present study represents the first report of sequence variants of the Ob-Rb gene in the Japanese and provides evidence against either obesity-causing mutations or association of sequence variants with obesity in obese Japanese subjects. [Diabetologia (1997) 40: 1204–1210]
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 47 (1982), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Several established methods of phytic acid determination in soybeans were evaluated. Iron analysis methods, which rely on a 4:6 molar ratio of Fe:P, were eliminated because this ratio was not dependable. Three assay methods relying on phosphorus analysis were then compared. The anion-exchange method was considered most accurate but not convenient for routine analysis. Analysis of the ferric phytate precipitate and a new method, analysis of the supernatant before and after ferric chloride precipitation, were judged against the anion-exchange method under different extraction conditions. Based upon good agreement with anion-exchange column results and acceptable reproducibility, the best methods were (1) precipitate analysis of phosphorus after extraction with 3% TCA + 10% sodium sulfate, or (2) the supernatant difference method after extraction with 1.2% HCl.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 54 (1989), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Egg white (EW) was desugared, adjusted to either pH 7 or pH 9, and freeze-dried. Portions were held at 25°, 50°, 60°, 70° or 80°C for 1 wk. In vitro iron bioavailability (IVIB) was estimated by the method of Kane and Miller (1984) and no differences in IVIB were observed due to EW pH. IVIB of EW held at 50°, 60°, or 70°C was not different (P〉0.05) than that of EW held at 25°C [9.87% dialyzable iron (DI)]; only the 80°C treatment led to IVIB lower (6.76% DI, P〈0.05) than the 25°C control. Differential scanning calorimetry and nondissociating electrophoresis showed that native protein structure, including that of the iron-binding protein ovotransferrin, was maintained after the 70°C treatment but not after the 80°C treatment.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-3091
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: In the northern parts of the Needwood and Stafford/Eccleshall Basins, England, the Pebble Beds of the Sherwood Sandstone Group contain thick successions of texturally mature, fluvial pebble/cobble conglomerates which are organized into either horizontal or cross-stratified sets. The horizontally lying sets, generally coarser grained and more poorly sorted than the cross-bedded sets, are usually disorganized and either matrix- or clast-supported, although thin lenses of well-sorted, occasionally openwork units, interpreted as falling stage phenomena, are often present. The cross-stratified conglomerates have foresets exhibiting remarkable textural organization, with a coarse, bimodal (sometimes matrix-supported) part grading upwards or being abruptly overlain by a finer, well-sorted (occasionally openwork) part and finally capped by sandstone. These rhythmic textural changes are attributed partly to an avalanche process at high stage and partly to falling stage conditions. The most common types of vertical association are thick successions of horizontally bedded conglomerates (up to 20 m) and sequences of an upwards coarsening nature (2-12 m) in which cross-stratified sets are overlain by flat-lying sets.The environment of deposition of the gravels is interpreted as one in which water depths at high stage were greater than depths in most modern braided stream plains (proglacial or alluvial fan) but shallower than depths associated with the Pleistocene catastrophic floods from which texturally mature, giant gravel bars have been recorded. Recent braided streams with relatively confined channels and considerable bar/channel relief are better analogues. In particular, medial or mid-channel bars with a two-tier structure (subaqueous and partly emergent portions) may explain the upward-coarsening sequences in which horizontally lying conglomerates overlie cross-stratified conglomerates. The thicker sequences of horizontally stratified conglomerates represent proximal, longitudinal bar deposits.Sheets of pebbly sandstone and argillaceous sandstone lying between the conglomerates, and commonly occurring towards the top of the succession, largely represent deposition from sandwaves and dunes. Finer, interbedded, argillaceous sandstones, siltstones and mudstones are interpreted as overbank and waning-flood deposits.Basin-forming tectonism of increasing intensity probably caused the initial coarsening upwards of the lower part of the succession, whilst more stable tectonic conditions and decreasing relief on the margins of the basins and in the areas of provenance in the Midlands and the Hercynides, account for the upwards-fining of the upper part of the succession.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 72 (1987), S. 77-82 
    ISSN: 1432-1939
    Keywords: Desert ; Rodent community ; Food addition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary From 1977 through 1983 we conducted experiments on a desert rodent community where supplemental seeds were added or certain rodent species and ants were removed from 0.25-ha fenced plots in a Chihuahuan Desert site in southeastern Arizona, USA. In this paper we examine the patterns of microhabitat use relative to vegetative cover by 11 rodent species. The results show that: i) removal of the largest seed-eating species, Dipodomys spectabilis, produced the most pervasive and dramatic shifts in microhabitat use by the remaining rodent species; ii) adding seeds or removing ants had little effect on the spatial use of microhabitats by rodents in this community; and iii) non-granivores were just as likely as granivores to shift microhabitat use when other granivores were removed. We believe these results indicate that both food and foraging microsites are limited but the relegation of subdominant species to lesspreferred microhabitats by the large Dipodomys spectabilis is the major factor underlying the spatial organization of this community. Results also demonstrate that strong interactions among species increase the probability that pathways of indirect interactions through intermediary species are important; these complex linkages may include species that overlap little in food preferences.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1939
    Keywords: Optimal foraging ; Nutrient constraints
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Observations of the foraging behavior of six captive dark-eyed juncos (Junco hyemalis) are used to test the assumptions and predictions of optimal diet choice models (Pyke et al. 1977) that include nutrients (Pulliam 1975). The birds sequentially encountered single seeds of niger thistle (Guizotia abyssinica) and of canary grass (Phalaris canariensis) on an artificial substrate in the laboratory. Niger thistle seeds were preferred by all birds although their profitability in terms of energy intake (J/s) was less than the profitability of canary grass seeds. Of four nutritional components used to calculate profitabilities (mg/s) lipid content was the only characteristic that could explain the junco's seed preference. As predicted by optimal diet theory the probability of consuming niger thistle seeds was independent of seed abundance. However, the consumption of 71–84% rather than 100% of the seeds encountered is not consistent with the prediction of all-or-nothing selection. Canary grass seeds were consumed at a constant rate (no./s) independent of the number of seeds encountered. This consumption pattern invalidates a model that assumes strict maximization. However, it is consistent with the assumption that canary grass seeds contain a nutrient which is required in minimum amounts to meet physiological demands (Pulliam 1975). These experiments emphasize the importance of incorporating nutrients into optimal foraging models and of combining seed preference studies with studies of the metabolic requirements of consumers.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Biodiversity and conservation 1 (1992), S. 179-208 
    ISSN: 1572-9710
    Keywords: alpine ; vegetation diversity ; habitat conservation ; arctic alpine vegetation ; grazing impacts ; snowbeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The montane (low- to mid-alpine) zone in Great Britain (GB) lies above the potential tree-line (700–800 m, but descending to 200 m in the north). It is composed of moss and lichen heaths, snowbeds, blanket bog and dwarf-shrub (Ericaceae) health-covered solifluction/gelifluction terraces (38 communities/sub-communities). Approximately 3.0% of the land surface is covered by this- the most extensive predominantly near-natural terrestrial habitat in GB. Internationally distinctive features include oceanic and southern biotic outliers of arctic-alpine fellfield and mountain tundra, and plant communities that are either globally rare/localised or especially well represented in GB. The absence of extensive sub-alpineBetula spp. andSalix spp. scrub is striking. The main sources of habitat diversity are climate, regional variation in topography and geology, and regional modifications due to land-use impact. Over 50 examples are given. Five important gradients in Scottish Highland vegetation are described. Only some 15% of the sampled montane vegetation is anthropogenic; the rest is semi- or near-natural. The vegetation is divided into 5 functional groups: chionophobous (avoids snow), chionophilous (prefers snow), species-rich, mires (including springs and flushes), and anthropogenic. Chionophobous and then chionophilous communities contribute most to montane vegetation diversity (calculated here as the ShannonH diversity index).H diversity increases asymptotically with montane site area but linearly with the number of communities present. A more varied topography, geology and topo-climate gives the highestH diversity. Two examples of montane biodiversity reductions south of the Highlands are the loss of prostrateCalluna vulgaris heaths and modification ofRacomitrium lanuginosum healths. Five objectives for nature conservation are proposed, covering restoration of montaneR. lanuginosum healths, prostrate dwarf-shrub dominated heaths, sub-alpine scrub and upper treelines, and the extension of the breeding ranges of both ptarmigan (Lagopus mutus) and dotterel (Charadrius morinellus) south of the Scottish Highlands. International support for monitoring is sought.
    Type of Medium: Electronic Resource
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