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Histamine content and release of isolated rat gastric mucosal cells

  • Histamine and Gastrointestinal Tract
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Abstract

Enzymatically dispersed rat gastric cells were subdivided (Percoll®) in fractions (F1, F2, F3) with different number of parietal cells (PC) and the intracellular histamine content was estimated (ng/106 cells): F1 (9% PC): 17±4 (SEM), F2 (26% PC): 80±9 and F3 (77% PC): 134±15. Histidinedecarboxylase showed the same pattern of distribution, F1 low, F2 medium, F3 high activity. Incubated F3 cells constantly released histamine (19±3 ng/106 cells/h), a process which could be stimulated by carbachol, forskolin and hexoprenaline. The data suggest: (1) rat gastric histaminocytes copurify with PC, (2) background stimulation by histamine should be considered in isolated cell systems, (3) vagal stimulation may release histamine within the gastric mucosa.

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität Tübingen, FRG

    H. -J. Ruoff

  2. Anatomisches Institut der Universität Tübingen, FGR

    K. Reutter

  3. Medizinische Poliklinik der Universität Bonn, FRG

    W. Schepp

Authors
  1. H. -J. Ruoff
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  2. K. Reutter
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  3. W. Schepp
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Ruoff, H.J., Reutter, K. & Schepp, W. Histamine content and release of isolated rat gastric mucosal cells. Agents and Actions 16, 202–204 (1985). https://doi.org/10.1007/BF01983139

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  • DOI: https://doi.org/10.1007/BF01983139

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