Abstract
The phorbol ester 12-O-tetradecanoyl-13-acetate (TPA) induced a dose-dependent release of histamine from rat peritoneal mast cells at concentrations from 3 to 100 ng/ml. The release is biphasic: an early phase being complete in 15 min and being followed by a second phase extending for more than 50 min. Concentrations of TPA greater than 100 ng/ml produced decreasing releases of histamine. Synergistic interaction in the induction of histamine secretion was observed between TPA and A23187 and between TPA and anti-IgE. Such synergism with anti-IgE was only manifest at low concentrations of TPA and with incubations of the cells with TPA for 5 mins or less. At higher concentrations of TPA and longer incubations, TPA inhibited the response of rat peritoneal mast cells to anti-IgE stimulation. Synergism between A23187 and TPA was observed only at low levels of histamine release induced by calcium plus A23187: at higher levels of release TPA was inhibitory.
Similar content being viewed by others
References
S. Cockroft andB.D. Gomperts,Evidence for a role of phosphatidyl inositol turnover in stimulus-secretion coupling, Biochem. J.178, 681–687 (1979).
M.J. Berridge andR.F. Irvine,Inositol triphosphate, a novel second messenger in cellular signal transduction, Nature312, 315–321 (1984).
J.F. Kuo, R.G.G. Anderson, B.C. Wise, L. Mackerlova, I. Salomonsson, N.L. Brackett, N. Katoh, M. Shoji andR.W. Wrenn,Calcium-dependent protein kinase: widespread occurrence in various tissues and phyla of the animal kingdom and comparison of effects of phospholipid, calmodulin and trifluoperazine, Proc. Natl. Acad. Sci. U.S.A.77, 7039–7043 (1980).
J.W. Putney, J.S. McKinney, D.L. Aub andB.A. Leslie,Phorbol ester-induced protein secretion in rat parotid gland. Relationship to the role of inositol lipip breakdown and protein kinase C activation in stimulussecretion coupling, Molec. Pharmacol.26, 261–266 (1984).
R. Sagi-Eisenberg, J.C. Foreman andR. Shelly,Histamine release induced by histone and phorbol ester from rat peritoneal mast cells, Eur. J. Pharmacol.113, 11–17 (1985).
R. Sagi-Eisenberg andI. Pecht,Protein kinase C, a coupling element between stimulus and secretion of basophils, Immun. Lett.8, 237–241 (1984).
R. Sagi-Eisenberg, H. Lieman andI. Pecht,Protein Kinase C regulation of the receptor-coupled calcium signal in histamine-secretory rat basophilic leukaemic cells, Nature313, 59–60 (1985).
Y. Katakami, K. Kabuchi, M. Sawamura, Y. Takai andY. Nishizuka,Synergistic action of protein kinase C and calcium for histamine release from rat peritoneal mast cells, Biochem. Biophys. Res. Commun.121, 573–578.
M. Castagna, Y. Takai, K. Kabuchi, K. Saro, U. Kikkawa andY. Nishizuka,Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters, J. Biol. Chem.257, 7847–7851 (1982).
T. Ishizaka, J.C. Foreman, A. Sterk andK. Ishizaka,Induction of calcium flux across the rat mast cell membrane by bridging IgE receptors, Proc. Natl. Acad. Sci. U.S.A.76, 5858–5862 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cantwell, M.E., Foreman, J.C. Characteristics of the effect of phorbol ester on rat mast cells: Interaction with anti-IgE. Agents and Actions 18, 77–80 (1986). https://doi.org/10.1007/BF01987988
Issue Date:
DOI: https://doi.org/10.1007/BF01987988