Abstract
Leucocytes from human peripheral blood were acid eluted and sensitizedin vitro with increasing concentrations of radiolabelled myeloma IgE. This sensitization step was performed with or without 30% IgE depleted serum. After the IgE binding, cells were washed and submitted to a challenge with monoclonal anti-IgE for the determination of the cellular sensitivity and reactivity in a histamine release assay. A sample of each of the sensitized cells was analyzed for its radioactivity and the number of basophils quantified, thus allowing the determination of the mean number of IgE molecules per basophil. Raising the IgE concentrations in the sensitization procedure led to an increase of the IgE on the basophil membrane, and to a concommitant elevation of the cell sensitivity. The presence of serum during the binding of IgE onto the cells lowers slightly the binding of IgE to the basophils but decreases strongly the cellular reactivity.
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References
L. M. Lichtenstein, P. S. Norman and W. L. Winkenwerder,Clinical and in vitro studies on the role of immunotherapy in ragweed hay fever. Am. J. Med.44, 514–524 (1968).
P. Stahl Skov, H. Permin and H. J. Malling,Quantitative and qualitative estimations of IgE bound to basophil leukocytes from hay fever patients. Scand. J. Immunol.6, 1021–1028 (1977).
A. Weyer, P. Ougen, J. P. Dandeu, F. Marchand and B. David,Passive in vitro sensitization of human basophils with dermatophagoides farinae specific IgE. Agents and Actions18, 178–181 (1986).
M. C. Conroy, N. F. Adkinson and L. M. Lichtenstein,Measurement of IgE on human basophils: relation to serum IgE and anti-IgE induced histamine release. J. Immunol.118, 1317–1321 (1977).
M. C. Conroy, M. A. Kings and A. L. de Weck,Anti-IgE-induced histamine release from human leukocytes. Fed. Proc.41, 376 (1982).
J. P. Bourgeois, M. Pere, J. C. Mazie and J. F. Delagneau,Monoclonal antibodies to human IgE: Utilization for total IgE quantification and estimation of allergen specific IgE antibodies. Develop. Biol. Standard, vol. 57, pp. 371–379 (S. Karger, Basel) (1984).
S. G. O. Johansson and H. Bennich,Immunological studies of an atypical (Myeloma) immunoglobulin. Immunology13, 381–394 (1967).
W. M. Hunter and F. C. Greenwood,Preparation of Iodine 131 labelled human growth hormone of high specific activity. Nature194, 495–496 (1962).
J. J. Pruzansky, L. C. Grammer, P. Patterson and M. Roberts,Dissociation of IgE from receptors on human basophils. I. Enhanced passive sensitization for histamine release. J. Immunol.131, 1949–1953 (1983).
L. M. Lichtenstein and D. W. MacGlashan jr.,The concept of basophil releasibility. J. Allergy clin. Immunol.77, 291–294 (1986).
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Weyer, A., Guesdon, J.L., Ougen, P. et al. Basophil sensitivity and reactivity to monoclonal anti-human IgE afterin vitro sensitization with human myeloma IgE. Agents and Actions 20, 210–212 (1987). https://doi.org/10.1007/BF02074671
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DOI: https://doi.org/10.1007/BF02074671