Abstract
Rat peritoneal mast cells were pretreated for 10 min at 37°C with either substance P (SP, 3 or 6 μM) or compound 48/80 (37.5, 50 or 75 ng/ml). The effect of this pretreatment on the subsequent responsiveness of the cells to SP was studied. Both SP and compound 48/80 pretreatment of rat peritoneal mast cells inhibited the subsequent response of the cells to SP. The degree of inhibition produced by either SP or compound 48/80 was dependent on the concentration used to pretreat the cells. Inhibition of the response of the cells to SP was observed whether or not the pretreating agent was removed or remained in contact with the cells during the subsequent stimulation with SP. It is concluded that compound 48/80 and SP desensitize the cells to subsequent stimulation by SP and possible mechanisms for this are discussed.
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References
B. Jasani, G. Kreil, B. F. Mackler and D. R. Stanworth,Further studies on the structural requirements for polypeptide mediated histamine release from rat mast cells, J. Biochem.181, 623–632 (1979).
C. M. S. Fewtrell, J. C. Foreman, C. C. Jordan, P. Oehme, H. Renner and J. M. Stewart,The effects of substance P on histamine and 5-hydroxytryptamine release in rat, J. Physiol.330, 393–411 (1982).
H. Repke, W. Piotrowski, M. Bienert and J. C. Foreman,Histamine release induced by Arg-Pro-Lys-Pro(CH 2)11CH3 from rat peritoneal mast cells, J. Pharmac. exp. Ther.,243, 317–322 (1987).
G. W. Read, S. M. Hong and E. F. Kiefer,Competitive inhibition of 48/80 induced histamine release by benzalkonium chloride and its analogs and the polyamine receptor in mast cells, J. Pharmac. exp. Ther.222, 652–657 (1982).
W. Piotrowski and J. C. Foreman,On the actions of substance P, somatostatin, and vasoactive intestinal polypeptide on rat peritoneal mast cells and in human skin. Naunyn Schmiedeberg's Arch. Pharmac.331, 364–368 (1985).
J. C. Foreman and J. L. Mongar,The role of the alkaline earth ions in anaphylactic histamine secretion, J. Physiol.224, 753–769 (1973).
F. Lembeck and G. Fischer,Gekreuzte Tachyphylaxie von Peptiden, Naunyn Schmiedeberg's Arch. Pharmac.258, 452–456 (1967).
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Dianzani, C., Foreman, J.C. Desensitization of rat peritoneal mast cells to substance P. Agents and Actions 23, 214–216 (1988). https://doi.org/10.1007/BF02142544
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DOI: https://doi.org/10.1007/BF02142544